Fluo-8 AM, green fluorescent calcium binding dye (ab142773)
Key features and details
- Medium affinity green fluorescent calcium binding dye. Cell-permeable.
- CAS Number: 1345980-40-6
Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Fluo-8 AM, green fluorescent calcium binding dye -
Description
Medium affinity green fluorescent calcium binding dye. Cell-permeable. -
CAS Number
1345980-40-6 -
Chemical structure
Properties
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Excitation
490nm -
Emission
520nm -
Chemical name
Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate
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Molecular weight
1046.94 -
Molecular formula
C50H50N2O23 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information. For more information on AM esters please visit our AM esters FAQ page.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c5ccccc5OCCOc1cc(ccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=2c4ccc(cc4OC3=CC(=O)C=CC=23)OCOC(C)=O -
Source
Synthetic
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Research areas
Images
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Chemical Structure - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)2D chemical structure image of ab142773, Fluo-8 AM, green fluorescent calcium binding dye
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Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)Cui et al PLoS One. 2013;8(3):e59921. doi: 10.1371/journal.pone.0059921. Epub 2013 Mar 20. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/FFA treatments increase Ca2+ influx to induce ERS in β-TC3 cells.
β-TC3 cells were incubated with FFA or BSA for 16 h, and then stimulated with 4 µM thapsigargin for 20 min to activate store-operated Ca2+ entry. Fluorescence densities of Ca2+ change were monitored in Fluo-8/AM-loaded β-TC3 cells after FFA or BSA treatments.
(From Figure 2B of Cui et al)
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Fluorescent Cell Imaging - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)U2OS cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom costar plate. The growth medium was removed, and the cells were incubated with 100 µl of 4 µM Fluo-4 AM or Fluo-8® AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 µl HHBS, then imaged with a fluorescence microscope using FITC channel.
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Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)Fluo-8 vs Fluo-4 sensitivity to calcium release in HEK-293 cells induced by Carbachol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab142773 has been referenced in 40 publications.
- Li C et al. Role of TRPV4 in the Diagnosis and Treatment of Helicobacter pylori Infection in Children with Duodenal Ulcers. Biomed Res Int 2022:2777882 (2022). PubMed: 35028313
- Eaton-Fitch N et al. Impaired TRPM3-dependent calcium influx and restoration using Naltrexone in natural killer cells of myalgic encephalomyelitis/chronic fatigue syndrome patients. J Transl Med 20:94 (2022). PubMed: 35172836
- Li Y et al. Songorine promotes cardiac mitochondrial biogenesis via Nrf2 induction during sepsis. Redox Biol 38:101771 (2021). PubMed: 33189984
- Zummo FP et al. Exendin-4 stimulates autophagy in pancreatic ß-cells via the RAPGEF/EPAC-Ca2+-PPP3/calcineurin-TFEB axis. Autophagy N/A:1-17 (2021). PubMed: 34338148
- Luo Q et al. Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage. Theranostics 11:5986-6005 (2021). PubMed: 33897894