Forskolin, Adenylyl cyclase activator (ab120058)
Key features and details
- Adenylyl cyclase activator
- CAS Number: 66575-29-9
- Soluble in ethanol to 10 mM and in DMSO to 25 mM
- Form / State: Solid
- Source: Coleus forskohlii
Overview
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Product name
Forskolin, Adenylyl cyclase activator -
Description
Adenylyl cyclase activator -
Biological description
Cell-permeable activator of adenylyl cyclase. Interacts directly with the catalytic subunit of the enzyme to increase intracellular cAMP levels. Positive inotropic, platelet antiaggregatory and antihypertensive actions in vivo. 3D Growth matrix component used in liver organoid generation.
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CAS Number
66575-29-9 -
Chemical structure
Properties
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Chemical name
(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-Dodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-3-vinyl-1H-benzo[f]chromen-5-yl acetate -
Molecular weight
410.50 -
Molecular formula
C22H34O7 -
PubChem identifier
47936 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 10 mM and in DMSO to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)O[C@H]2[C@@H](O)[C@@H]1[C@](C)([C@@H](O)CCC1(C)C)[C@@]3(O)C(=O)C[C@](C)(C=C)O[C@]23C -
Source
Coleus forskohlii
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Research areas
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Biochemicals
- Research Area
- Heart disease
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Pain & inflammation
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Respiratory disease
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
Images
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Chemical Structure - Forskolin, Adenylyl cyclase activator (ab120058)2D chemical structure image of ab120058, Forskolin, Adenylyl cyclase activator
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Functional Studies - Forskolin, Adenylyl cyclase activator (ab120058)Gambino et al PLoS One. 2010 Jul 2;5(7):e11399. doi: 10.1371/journal.pone.0011399. Fig S4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Effect of AC activation onto adult Mecp2X/Y and Mecp2308/Y Cortico-LA synapses.
A: A 10 minute application of the AC activator Forskolin (FSK, 50 µM) potentiates the Cortico-LA EPSC in young WT animals.
B: The same protocol is inefficient in adult WT mice (B1) and Mecp2308/Y mice (B2).
C: Summary plot of similar pharmacological experiments in WT and KO mice.
***: P<0.001.
Number of recorded cells is indicated.
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Functional Studies - Forskolin, Adenylyl cyclase activator (ab120058)ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
Functional Studies - Forskolin, Adenylyl cyclase activator (ab120058)1e7 3T3-L1 cells were treated for 15 minutes with 1 µM dexamethasone (ab120743) and 1 µg x mL-1 insulin (ab123768) with the addition of 50 µM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424), extracted according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (38)
ab120058 has been referenced in 38 publications.
- Ilhan R et al. Novel regulation mechanism of adrenal cortisol and DHEA biosynthesis via the endogen ERAD inhibitor small VCP-interacting protein. Sci Rep 12:869 (2022). PubMed: 35042898
- Miete C et al. Gai2-induced conductin/axin2 condensates inhibit Wnt/ß-catenin signaling and suppress cancer growth. Nat Commun 13:674 (2022). PubMed: 35115535
- Jia Q et al. Novel GSK-3 kinase inhibitor Pym-5 induces GSK-3β rather than GSK-3α-dependent melanogenesis in murine melanoma cells. J Dermatol Sci 106:170-180 (2022). PubMed: 35641396
- Xiong G et al. FOSL1 promotes tumor growth and invasion in ameloblastoma. Front Oncol 12:900108 (2022). PubMed: 36185257
- Sakai Y et al. BRCA1-BARD1 Regulates Axon Regeneration in Concert with the Gqa-DAG Signaling Network. J Neurosci 41:2842-2853 (2021). PubMed: 33593852