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    products/biochemicals/ionomycin-free-acid-ca2-ionophore-ab120370.pdf

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Biochemicals Chemical Type Biochemicals
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Ionomycin (free acid), Ca2+ ionophore (ab120370)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (2)References (7)

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Chemical Structure - Ionomycin (free acid), Ca<sup>2+</sup> ionophore (ab120370)
  • Functional Studies - Ionomycin (free acid), Ca<sup>2+</sup> ionophore (ab120370)

Key features and details

  • Ca2+ ionophore
  • CAS Number: 56092-81-0
  • Soluble in ethanol to 10 mM and in DMSO to 10 mM
  • Form / State: Liquid
  • Source: Streptomyces conglobatus

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Overview

  • Product name

    Ionomycin (free acid), Ca2+ ionophore
  • Description

    Ca2+ ionophore
  • Biological description

    Potent, highly selective Ca2+ ionophore. Effective mobile ion carrier for Ca2+, highly specific for divalent cations (Ca > Mg >> Sr = Ba). Useful for studies of Ca2+ transport across biological membranes and increasing intracellular Ca2+ concentrations. Complexes with Ca2+ between pH 7 and 9.5. Absorption max. (280 nm, MeOH): 18600 M-1cm-1.

  • CAS Number

    56092-81-0
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid
  • Molecular weight

    709.01
  • Molecular formula

    C41H72O9
  • PubChem identifier

    6912226
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in ethanol to 10 mM and in DMSO to 10 mM
  • Handling

    This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    O=C(O)CC[C@@H](C)C[C@H](C)C[C@H](C)C(=O)C=C(/O)[C@H](C)C[C@H](C)CC=C[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]1CC[C@](C)(O1)[C@H]2CC[C@](C)(O2)[C@@H](C)O
  • Source

    Streptomyces conglobatus

  • Research areas

    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Ion Channels
    • Ionophores, Indicators & Chelators
    • Biochemicals
    • Pharmacology
    • Signaling
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    • Biochemicals
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Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Ionomycin (free acid), Ca<sup>2+</sup> ionophore (ab120370)
    Chemical Structure - Ionomycin (free acid), Ca2+ ionophore (ab120370)
    2D chemical structure image of ab120370, Ionomycin (free acid), Ca2+ ionophore
  • Functional Studies - Ionomycin (free acid), Ca<sup>2+</sup> ionophore (ab120370)
    Functional Studies - Ionomycin (free acid), Ca2+ ionophore (ab120370)
    ab58668 staining ATF3 in A549 cells treated with ionomycin (free acid) (ab120370), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin (free acid), as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370 (Ionomycin (free acid)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (7)

Publishing research using ab120370? Please let us know so that we can cite the reference in this datasheet.

ab120370 has been referenced in 7 publications.

  • Lu Q  et al. SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2. Immunity 54:1304-1319.e9 (2021). PubMed: 34048708
  • Usai C  et al. The ß-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19. Front Immunol 12:813300 (2021). PubMed: 35095908
  • Liu YJ  et al. Parthenolide ameliorates colon inflammation through regulating Treg/Th17 balance in a gut microbiota-dependent manner. Theranostics 10:5225-5241 (2020). PubMed: 32373209
  • Lautenschläger J  et al. Intramitochondrial proteostasis is directly coupled to a-synuclein and amyloid ß1-42 pathologies. J Biol Chem 295:10138-10152 (2020). PubMed: 32385113
  • Grau-Expósito J  et al. A Novel Single-Cell FISH-Flow Assay Identifies Effector Memory CD4(+) T cells as a Major Niche for HIV-1 Transcription in HIV-Infected Patients. MBio 8:N/A (2017). Human . PubMed: 28698276
  • Puskarjov M  et al. Activity-dependent cleavage of the K-Cl cotransporter KCC2 mediated by calcium-activated protease calpain. J Neurosci 32:11356-64 (2012). PubMed: 22895718
  • Weingärtner A  et al. Ca2+-activated transbilayer movement of plasma membrane phospholipids in Leishmania donovani during ionomycin or thapsigargin stimulation. Mol Biochem Parasitol 179:59-68 (2011). PubMed: 21684309

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Dear Supplier,
I am looking to find out which is the more suitable product for me to be using between https://www.abcam.com/ionomycin-ca2-salt-ab120116.html and https://www.abcam.com/ionomycin-free-acid-ab120370.html . I will be using it in cell culture of human cancer cell lines to induce calcium influx. Any help with choosing the more appropriate product would be greatly appreciated.
Thanks

Read More

Abcam community

Verified customer

Asked on Feb 22 2013

Answer

Thank you for contacting us.

Product ab120116 (Ionomycin Ca2+ Salt) differs from ab120370 (free acid), as the presence of the Ca2+ makes the compound easier to solubilise (the free acid is soluble in ethanol to 10 mM and in DMSO to 10 mM. The Ca2+ salt is soluble in DMSO to 25 mM and in ethanol to 100 mM).

Apart from this, it may depend on your specific experimental requirements. Some researches prefer not to use the Ca2+ salt as they prefer not to introduce extra calcium into the system.

I hope this information was helpful. Please do not hesitate to contact us should you require further assistance.

Read More

Abcam Scientific Support

Answered on Feb 22 2013

Question

Ich habe den WB-Fragebogen ausgefuellt, ich hoffe Sie koennen mir helfen.

Read More

Abcam community

Verified customer

Asked on Mar 07 2012

Answer

Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Die Bande bei 50 kDa sieht in der Tat unspezifisch aus; dies wäre allerdings ja nicht weiter schlimm, wenn Sie die spezifische Bande bei 120/140 kDa detektieren würden. Ihr Protokoll sieht absolut in Ordnung aus, und daher macht es mir etwas Sorgen, dass Sie keine positiven Ergebnisse erzielen.

Um der Sache auf den Grund zu gehen, würde ich Sie gerne noch nach einigen Details fragen:

Wie haben Sie die Jurkat-Zellen infiziert (Vpr-/wt/M)? Und wissen Sie, ob diese Infektion den T-Zellrezeptorkomplex aktiviert und damit die Expression von NFAT induziert?

Oder anders gefragt: Haben Sie eine Phorbol 12-myristate 13-acetate (PMA)/Ionomycin-stimulierte Positivkontrolle mitgeführt? Sollte der Antikörper mit dieser Kontrolle auch keine Bande für NFAT ergeben, wäre das der Beweis, dass mit dem Vial etwas nicht stimmt, und ich könnte Ihnen umgehend einen kostenlosen Ersatz zusenden. Wir haben zum einen PMA und Ionomycin in unserem Katalog, falls Sie Ihre Zellen selbst stimulieren möchten (ab120297, ab120116, ab120370), oder wir haben Lysate stimulierter Zellen wie zum Beispiel ab14850 oder ab14843 verfügbar.

Click here (or use the following: https://www.abcam.com/Phorbol-12-myristate-13-acetate-PMA-PKC-activator-ab120297.html).
Click here (or use the following: https://www.abcam.com/Ionomycin-Ca2-Salt-Ca2-ionophore-ab120116.html).
Click here (or use the following: https://www.abcam.com/Ionomycin-free-acid-Ca2-ionophore-ab120370.html).
Click here (or use the following: https://www.abcam.com/Jurkat-nuclear-extract-lysate-TPA--CI-stimulated-ab14850.html).
Click here (or use the following: https://www.abcam.com/Jurkat-Human-Cytoplasmic-Lysate-TPA-CI-stimulated-ab14843.html).

Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich auf Ihre Antworten.

Read More

Abcam Scientific Support

Answered on Mar 07 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

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