L-Glutamate, excitatory neurotransmitter (ab120049)
Key features and details
- Excitatory neurotransmitter
- CAS Number: 56-86-0
- Soluble in 1 eq. NaOH to 100 mM and in water to 25mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
L-Glutamate, excitatory neurotransmitter -
Description
Excitatory neurotransmitter -
Biological description
Major excitatory neurotransmitter. Acts at glutamate receptors. Active in vivo.
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CAS Number
56-86-0 -
Chemical structure
Properties
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Chemical name
(S)-2-Aminopentanedioic acid -
Molecular weight
147.13 -
Molecular formula
C5H9NO4 -
PubChem identifier
33032 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in 1 eq. NaOH to 100 mM and in water to 25mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
OC(=O)CC[C@H](N)C(=O)O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - L-Glutamate, excitatory neurotransmitter (ab120049)2D chemical structure image of ab120049, L-Glutamate, excitatory neurotransmitter
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Immunocytochemistry/ Immunofluorescence - L-Glutamate, excitatory neurotransmitter (ab120049)ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (4)
ab120049 has been referenced in 4 publications.
- Guo C et al. Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex. Elife 10:N/A (2021). PubMed: 34369877
- Mukherjee C et al. Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain. Cell Metab 32:259-272.e10 (2020). PubMed: 32531201
- Cheng K et al. Calsyntenin-1 Negatively Regulates ICAM5 Accumulation in Postsynaptic Membrane and Influences Dendritic Spine Maturation in a Mouse Model of Fragile X Syndrome. Front Neurosci 13:1098 (2019). PubMed: 31680833
- Pergner J & Kozmik Z Amphioxus photoreceptors - insights into the evolution of vertebrate opsins, vision and circadian rhythmicity. Int J Dev Biol 61:665-681 (2017). PubMed: 29319115