Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)
Key features and details
- Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
- CAS Number: 87081-35-4
- Supplied in ethanol (25 μg/ml)
- Form / State: Liquid
- Source: Steptomyces sp.
Overview
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Product name
Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. -
Description
Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. -
Biological description
Potent inhibitor of CRM-1-mediated (exportin-1) nuclear export. Displays antibiotic, antifungal, and antitumor properties and blocks the cell cycle at G1 and G2 phases. Potent inhibitor of nucleo-cytoplasmic translocation of Rev. Also shown to suppress HIV-1 replication in human monocytes (IC50 = 600 pM).
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General notes
Supplied in ethanol (25 µg/ml).
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CAS Number
87081-35-4 -
Chemical structure
Properties
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Chemical name
(2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-[(2S,3S)-3,6-Dihydro-3-methyl-6-oxo-2H-pyran-2-yl]-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18-nonadecapentaenoic acid -
Molecular weight
540.74 -
Molecular formula
C33H48O6 -
PubChem identifier
6917907 -
Storage instructions
Store at -20°C (desiccating conditions). -
Solubility overview
Supplied in ethanol (25 μg/ml) -
Handling
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(O)C=C(/C)C[C@H](C)[C@@H](O)[C@H](C)C(=O)[C@H](C)C=C(/C)C=CC[C@@H](C)/C=C(C=C[C@@H]1OC(=O)C=C[C@@H]1C)CC -
Source
Steptomyces sp.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)2D chemical structure image of ab120501, Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
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Functional Studies - Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)ab38335 staining p75 in HeLa cells treated with Leptomycin B (ethanol solution) (ab120501), by ICC/IF. Increase in p75 expression in the nucleous and cytoplasm correlates with increased concentration of Leptomycin B (ethanol solution), as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120501 (Leptomycin B (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38335 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab120501 has been referenced in 2 publications.
- John SP et al. IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages. Cell Rep 25:95-106.e6 (2018). PubMed: 30282041
- Özdas S & Özdas T Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines. Turk J Biol 42:132-143 (2018). PubMed: 30814875