Metformin hydrochloride, AMPK activator (ab120847)
Key features and details
- AMPK activator
- CAS Number: 1115-70-4
- Purity: > 99%
Soluble in water to 100 mM and in DMSO to 50 mM.
Sonication and heating in a water bath to approximately 45 °C are required to produce a clear solution in DMSO.
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Metformin hydrochloride, AMPK activator -
Description
AMPK activator -
Biological description
AMPK activator. Antidiabetic and anticancer activity in vivo. Rapidly crosses the blood-brain barrier.
Also available in simple stock solutions (ab146725) - add 1 ml of water to get an exact, ready-to-use concentration. -
Purity
> 99% -
CAS Number
1115-70-4 -
Chemical structure
Properties
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Chemical name
N,N-Dimethylimidodicarbonimidic diamide hydrochloride
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Molecular weight
165.63 -
Molecular formula
C4H11N5.HCl -
PubChem identifier
14219 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM and in DMSO to 50 mM.
Sonication and heating in a water bath to approximately 45 °C are required to produce a clear solution in DMSO.
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CN(C)C(=N)N=C(N)N.Cl -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Metformin hydrochloride, AMPK activator (ab120847)2D chemical structure image of ab120847, Metformin hydrochloride, AMPK activator
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Immunocytochemistry/ Immunofluorescence - Metformin hydrochloride, AMPK activator (ab120847)ab1 staining HIF1α in MCF7 cells treated with metformin hydrochloride (ab120847), by ICC/IF. Decrease in HIF1α expression correlates with increased concentration of metformin hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (10)
ab120847 has been referenced in 10 publications.
- Rai D et al. Evaluation of photoreceptor-directed fibroblasts derived from retinitis pigmentosa patients with defects in the EYS gene: a possible cost-effective cellular model for mechanism-oriented drug. Stem Cell Res Ther 13:157 (2022). PubMed: 35410372
- Farfan-Morales CN et al. The antiviral effect of metformin on zika and dengue virus infection. Sci Rep 11:8743 (2021). PubMed: 33888740
- Ruparelia AA et al. Metformin rescues muscle function in BAG3 myofibrillar myopathy models. Autophagy 17:2494-2510 (2021). PubMed: 33030392
- Kundu A et al. 14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation. J Biol Chem 295:1754-1766 (2020). PubMed: 31901078
- Zhao Y et al. NEAT1 regulates microtubule stabilization via FZD3/GSK3ß/P-tau pathway in SH-SY5Y cells and APP/PS1 mice. Aging (Albany NY) 12:23233-23250 (2020). PubMed: 33221742
- Gong Y et al. Metformin Inhibits Tumor Metastasis through Suppressing Hsp90a Secretion in an AMPKa1-PKC? Dependent Manner. Cells 9:N/A (2020). PubMed: 31936169
- Wimmer RA et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565:505-510 (2019). PubMed: 30651639
- Blackmore K et al. LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice. Exp Biol Med (Maywood) 242:1490-1498 (2017). PubMed: 28766983
- Soto-Acosta R et al. DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog 13:e1006257 (2017). PubMed: 28384260
- Zhou Z et al. Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NF?B Pathway Suppression. J Diabetes Res 2016:4847812 (2016). PubMed: 27761470