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NBQX, AMPA / kainate antagonist (ab120045)

Submit a review Q&A (3)References (131)
Chemical Structure - NBQX, AMPA / kainate antagonist (ab120045)
  • Functional Studies - NBQX, AMPA / kainate antagonist (ab120045)
  • Functional Studies - NBQX, AMPA / kainate antagonist (ab120045)

Key features and details

  • AMPA / kainate antagonist
  • CAS Number: 118876-58-7
  • Soluble in DMSO to 100 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    NBQX, AMPA / kainate antagonist

  • Description

    AMPA / kainate antagonist

  • Biological description

    Potent, selective and competitive AMPA/kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo. Water-soluble form available - please see NBQX disodium salt (ab120046).

  • CAS Number

    118876-58-7

  • Chemical structure

    Chemical Structure

Properties

Images

  • Chemical Structure - NBQX, AMPA / kainate antagonist (ab120045)
    Chemical Structure - NBQX, AMPA / kainate antagonist (ab120045)
    2D chemical structure image of ab120045, NBQX, AMPA / kainate antagonist
  • Functional Studies - NBQX, AMPA / kainate antagonist (ab120045)
    Functional Studies - NBQX, AMPA / kainate antagonist (ab120045)Barker M et al. PLoS One. 2012; 7(5): e35955. 10.1371/journal.pone.0035955 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    A - Photomicrograph of a DCN fusiform cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this fusiform cell while stimulating the LVN (bottom). 

    B - Photomicrograph of a DCN granule cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this granule cell while stimulating the LVN (bottom)

    Both cells were held at −68 mV and the LVN was stimulated at 0.3 Hz. Glutamatergic EPSCs are represented in black and are blocked by 50 µm D-AP5 and 10 µm NBQX (ab120045, traces in red). Each trace represents an average of 10–20 single traces. The arrowhead represents the artifact of stimulus that has been removed for clarity. Scale bar: (A) 50 µm, (B) 20 µm

     

    Credit: Barker M et al. PLoS One. 2012; 7(5): e35955. 10.1371/journal.pone.0035955

  • Functional Studies - NBQX, AMPA / kainate antagonist (ab120045)
    Functional Studies - NBQX, AMPA / kainate antagonist (ab120045)
    ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (131)

Publishing research using ab120045? Please let us know so that we can cite the reference in this datasheet.

ab120045 has been referenced in 131 publications.

  • Vaden RJ  et al. Parvalbumin interneurons provide spillover to newborn and mature dentate granule cells. Elife 9:N/A (2020). PubMed: 32602839
  • Goz RU  et al. BRAFV600E expression in neural progenitors results in a hyperexcitable phenotype in neocortical pyramidal neurons. J Neurophysiol 123:2449-2464 (2020). PubMed: 32401131
  • Vaden JH  et al. The readily-releasable pool dynamically regulates multivesicular release. Elife 8:N/A (2019). PubMed: 31364987
  • Lu C  et al. Overexpression of NEUROG2 and NEUROG1 in human embryonic stem cells produces a network of excitatory and inhibitory neurons. FASEB J 33:5287-5299 (2019). PubMed: 30698461
  • Kitko KE  et al. Membrane cholesterol mediates the cellular effects of monolayer graphene substrates. Nat Commun 9:796 (2018). PubMed: 29476054
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

That is very kind of you to do so.
The date of purchase was the ******* (although it may come up slightly
later because of the ordering system of the univeristy, I order and then it
may take a day or two to be authorised and then ordered). The reference
order will hopefully either be ****** or *******
Thank you for you help and service.

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Answer

Thank you for confirming these details and for your cooperation.

As agreed, I have issued a free of charge replacement with the order number ****.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question

Thank you for confirming this for me. I made up a solution you see in H2O and it did not look like it disolved as I followed the wording on the front of the delievery package rather than the front. So thought best to check!

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Answer

Thank you for contacting us.

Sorry again for the wrong information provided for the reconstitution of NBQX ab120045. Iwould be pleased to send you a free of charge unit of NBQX ab120045 in compensation.In order to do so, could you please send me one of the following : the order number, the PO number, the date of purchase or at least the address of your lab?

I look forward to your reply.

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Question

Hi,
I am enquiring to see whether NBQX can be dissolved and stored in H2O?
As on the info that came with the packaging, under the product constituents
and storage it states it is soluble in water to 100mM. However, at the end
of the info sheets, in the certificate of analysis section it states it is
soluble in DMSO to 100mM. I do see that you also sell a water-soluble form,
however I'm a little confused to whether the NBQX that I am enquiring about
is also soluble in water?
If you could clear this up that would be great.

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Answer

Thank you for contacting us.

I have checked with the lab, and we can confirm that this compound NBQX ab120045 is not soluble in water and is actually only soluble in DMSO to 100 mM.Thiswas a mistake in our database which has now been updated.

If you require the water soluble form of NBXQ we would recommend ab120046.

We sincerely apologize for this mistake and any inconvenience this might have caused. We thank you for bringing this to our attention.Please do not hesitate to contact us if you have further questions in this regard.

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