NBQX disodium salt, AMPA / kainate antagonist (ab120046)
Key features and details
- AMPA / kainate antagonist; water soluble
- CAS Number: 479347-86-9
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
NBQX disodium salt, AMPA / kainate antagonist -
Description
AMPA / kainate antagonist; water soluble -
Biological description
Water soluble, potent, selective and competitive AMPA / kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo.
Also available in simple stock solutions (ab144489) - add 1 ml of water to get an exact, ready-to-use concentration. -
CAS Number
479347-86-9 -
Chemical structure
Properties
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Chemical name
2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt -
Molecular weight
380.24 -
Molecular formula
C12H6N4Na2O6S -
PubChem identifier
3272523 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Na+].[Na+].NS(=O)(=O)c3cccc2c3c(cc1nc([O-])c([O-])nc12)[N+]([O-])=O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - NBQX disodium salt, AMPA / kainate antagonist (ab120046)2D chemical structure image of ab120046, NBQX disodium salt, AMPA / kainate antagonist
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Functional Studies - NBQX disodium salt, AMPA / kainate antagonist (ab120046)ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX disodium salt (ab120046), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX disodium salt, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120046 (NBQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (51)
ab120046 has been referenced in 51 publications.
- Wei XP et al. A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing. Neuron 110:644-657.e6 (2022). PubMed: 34998469
- Prakash M et al. Selective control of synaptically-connected circuit elements by all-optical synapses. Commun Biol 5:33 (2022). PubMed: 35017641
- Horvath PM et al. A subthreshold synaptic mechanism regulating BDNF expression and resting synaptic strength. Cell Rep 36:109467 (2021). PubMed: 34348149
- Guo C et al. Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex. Elife 10:N/A (2021). PubMed: 34369877
- Balmer TS et al. Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse. Elife 10:N/A (2021). PubMed: 33616036