Nimodipine, L-type Ca2+ channel blocker (ab120138)
Key features and details
- L-type Ca2+ channel blocker
- CAS Number: 66085-59-4
- Soluble in DMSO to 100 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Nimodipine, L-type Ca2+ channel blocker -
Description
L-type Ca2+ channel blocker -
Biological description
L-type Ca2+ channel blocker. Potent cerebrovasodilator. Cognitive enhancer. More lipophilic than nifedipine (ab120135).
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General notes
Nimodipine is light sensitive and it is recommended that the compound is protected from light.
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CAS Number
66085-59-4 -
Chemical structure
Properties
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Chemical name
1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylic acid 2-methoxyethyl 1-methylethyl ester -
Molecular weight
418.44 -
Molecular formula
C21H26N2O7 -
PubChem identifier
4497 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC=1NC(C)=C(C(C=1C(=O)OC(C)C)c2cccc(c2)[N+]([O-])=O)C(=O)OCCOC -
Source
Synthetic
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Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Nimodipine, L-type Ca2+ channel blocker (ab120138)2D chemical structure image of ab120138, Nimodipine, L-type Ca2+ channel blocker
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Functional Studies - Nimodipine, L-type Ca2+ channel blocker (ab120138)ab2770 staining aryl hydrocarbon receptor in MDA-MB-231 cells treated with nimodipine (ab120138), by ICC/IF. Increase in aryl hydrocarbon receptor expression correlates with increased concentration of nimodipine, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120138 (nimodipine) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2770 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (4)
ab120138 has been referenced in 4 publications.
- Pousinha PA et al. The Amyloid Precursor Protein C-Terminal Domain Alters CA1 Neuron Firing, Modifying Hippocampus Oscillations and Impairing Spatial Memory Encoding. Cell Rep 29:317-331.e5 (2019). PubMed: 31597094
- Han KS et al. Ephaptic Coupling Promotes Synchronous Firing of Cerebellar Purkinje Cells. Neuron 100:564-578.e3 (2018). PubMed: 30293822
- Cohen SM et al. Evolutionary and functional perspectives on signaling from neuronal surface to nucleus. Biochem Biophys Res Commun 460:88-99 (2015). PubMed: 25998737
- Wheeler DG et al. Ca(V)1 and Ca(V)2 channels engage distinct modes of Ca(2+) signaling to control CREB-dependent gene expression. Cell 149:1112-24 (2012). PubMed: 22632974