Nocodazole, Microtubule inhibitor (ab120630)
Key features and details
- Microtubule inhibitor
- CAS Number: 31430-18-9
- Purity: > 98%
Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Nocodazole, Microtubule inhibitor -
Description
Microtubule inhibitor -
Purity
> 98% -
CAS Number
31430-18-9 -
Chemical structure
Properties
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Chemical name
Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate
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Molecular weight
301.32 -
Molecular formula
C14H11N3O3S -
PubChem identifier
4122 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 50 mM
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3 -
Source
Synthetic
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Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Images
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Chemical Structure - Nocodazole, Microtubule inhibitor (ab120630)2D chemical structure image of ab120630, Nocodazole, Microtubule inhibitor
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Western blot - Nocodazole, Microtubule inhibitor (ab120630)All lanes : Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 23 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunoprecipitation - Nocodazole, Microtubule inhibitor (ab120630)Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate 10 μg (Input).
Lane 2: ab217673 IP in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab120630 has been referenced in 11 publications.
- Lee BY & Hur EM A Role of Microtubules in Oligodendrocyte Differentiation. Int J Mol Sci 21:N/A (2020). PubMed: 32033476
- Wu B et al. Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells. Cell Metab 32:967-980.e5 (2020). PubMed: 33264602
- Sugita S et al. Tumor-suppressive microRNA-223 targets WDR62 directly in bladder cancer. Int J Oncol 54:2222-2236 (2019). PubMed: 30942440
- Ocampo J et al. Contrasting roles of the RSC and ISW1/CHD1 chromatin remodelers in RNA polymerase II elongation and termination. Genome Res 29:407-417 (2019). PubMed: 30683752
- Tabdanov ED et al. Bimodal sensing of guidance cues in mechanically distinct microenvironments. Nat Commun 9:4891 (2018). PubMed: 30459308