PD 173074, FGFR1 and FGFR3 inhibitor (ab141117)
Key features and details
- Potent FGFR1 and FGFR3 inhibitor
- CAS Number: 219580-11-7
- Purity: > 99%
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
PD 173074, FGFR1 and FGFR3 inhibitor -
Description
Potent FGFR1 and FGFR3 inhibitor -
Purity
> 99% -
CAS Number
219580-11-7 -
Chemical structure
Properties
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Chemical name
N-[2-[[4-(Diethylamino)butyl]amino]-6-(3,5-dimethoxyphenyl)pyrido[2,3-d]pyrimidin-7-yl]-N'-(1,1-dimethylethyl)urea -
Molecular weight
523.68 -
Molecular formula
C28H41N7O3 -
PubChem identifier
1401 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(C)(C)NC(=O)Nc1nc3nc(NCCCCN(CC)CC)ncc3cc1c2cc(OC)cc(OC)c2 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - PD 173074, FGFR1 and FGFR3 inhibitor (ab141117)2D chemical structure image of ab141117, PD 173074, FGFR1 and FGFR3 inhibitor
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Functional Studies - PD 173074, FGFR1 and FGFR3 inhibitor (ab141117)BT549 cells were incubated at 37ºC for 1 hour with vehicle control (0 µM) and different concentrations of PD 173074 (ab141117) in DMSO. Decreased expression of RSK1 p90 (phospho T359 + S363) ab32413 correlates with an increase in PD 173074 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32413 at 1/5000 dilution and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP ab97051 at 1/10000 dilution and visualised using ECL development solution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab141117 has been referenced in 2 publications.
- Decker B et al. Global Genome Conformational Programming during Neuronal Development Is Associated with CTCF and Nuclear FGFR1-The Genome Archipelago Model. Int J Mol Sci 22:N/A (2020). PubMed: 33396256
- Yu Y et al. The G-Protein-Coupled Chemoattractant Receptor Fpr2 Exacerbates High Glucose-Mediated Proinflammatory Responses of Müller Glial Cells. Front Immunol 8:1852 (2017). PubMed: 29312335