PD 98059, MEK1 inhibitor (ab120234)
Key features and details
- MEK1 inhibitor
- CAS Number: 167869-21-8
- Soluble in DMSO to 25 mM and in ethanol to 5 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
PD 98059, MEK1 inhibitor -
Description
MEK1 inhibitor -
Alternative names
- PD090859
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Biological description
Inhibitor of MKK1 (MEK1).
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CAS Number
167869-21-8 -
Chemical structure
Properties
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Chemical name
2-(2-Amino-3-methoxyphenyl)-4H-1-benzopyran-4-one -
Molecular weight
267.28 -
Molecular formula
C16H13NO3 -
PubChem identifier
4713 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 25 mM and in ethanol to 5 mM -
Handling
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COc3cccc(C1=CC(=O)c2ccccc2O1)c3N -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - PD 98059, MEK1 inhibitor (ab120234)2D chemical structure image of ab120234, PD 98059, MEK1 inhibitor
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Immunocytochemistry/ Immunofluorescence - PD 98059, MEK1 inhibitor (ab120234)ab18195 staining hnRNP K in HeLa cells treated with PD 98059 (ab120234), by ICC/IF. Changes in localization of hnRNP K (translocation from cytoplasm to nucleous) correlates with increased concentration of hnRNP K, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120234 ( hnRNP K) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18195 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (22)
ab120234 has been referenced in 22 publications.
- Zhang J et al. TGF-ß1-induced autophagy activates hepatic stellate cells via the ERK and JNK signaling pathways. Int J Mol Med 47:256-266 (2021). PubMed: 33236148
- Li HN et al. Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-a through activating ERK, p38 MAPK, and JAK2/STAT3. J Neuroinflammation 18:150 (2021). PubMed: 34225736
- Lanfranco MF et al. Expression and secretion of apoE isoforms in astrocytes and microglia during inflammation. Glia 69:1478-1493 (2021). PubMed: 33556209
- Nunes JPS et al. Co-Exposure of Cardiomyocytes to IFN-? and TNF-a Induces Mitochondrial Dysfunction and Nitro-Oxidative Stress: Implications for the Pathogenesis of Chronic Chagas Disease Cardiomyopathy. Front Immunol 12:755862 (2021). PubMed: 34867992
- Fukushima T et al. Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium. Cell Rep 29:4144-4158.e7 (2019). PubMed: 31851939