Pentoxifylline, Phosphodiesterase and cytokine inhibitor (ab120725)
Key features and details
- Phosphodiesterase inhibitor. Cytokine inhibitor.
- CAS Number: 6493-05-6
- Purity: > 99%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Pentoxifylline, Phosphodiesterase and cytokine inhibitor -
Description
Phosphodiesterase inhibitor. Cytokine inhibitor. -
Alternative names
- oxpentifylline
- PTX
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Biological description
Phosphodiesterase inhibitor. Inhibits synthesis of TNF-α. Broad-spectrum inhibitor of cytokines. Displays anti-inflammatory and antinociceptive properties.
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Purity
> 99% -
CAS Number
6493-05-6 -
Chemical structure
Properties
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Chemical name
3,7-Dimethyl-1-(5-oxohexyl)xanthine -
Molecular weight
278.31 -
Molecular formula
C13H18N4O3 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Pentoxifylline, Phosphodiesterase and cytokine inhibitor (ab120725)2D chemical structure image of ab120725, Pentoxifylline, Phosphodiesterase and cytokine inhibitor
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Immunocytochemistry/ Immunofluorescence - Pentoxifylline, Phosphodiesterase and cytokine inhibitor (ab120725)ab54775 staining annexin V in HeLa cells treated with pentoxifylline (ab120725), by ICC/IF. Increase of annexin V correlates with increased concentration of pentoxifylline, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120725 (pentoxifylline) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab54775 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab120725 has been referenced in 2 publications.
- Basnet RM et al. Zebrafish Embryo as an In Vivo Model for Behavioral and Pharmacological Characterization of Methylxanthine Drugs. Int J Mol Sci 18:N/A (2017). PubMed: 28282918
- Basnet RM et al. Methylxanthines induce structural and functional alterations of the cardiac system in zebrafish embryos. BMC Pharmacol Toxicol 18:72 (2017). PubMed: 29141695