Pitstop® 2 - negative control, Negative control for clathrin inhibitor Pitstop® 2 (ab120688)
Key features and details
- Negative control for clathrin inhibitor Pitstop® 2 (ab120687)
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Pitstop® 2 - negative control, Negative control for clathrin inhibitor Pitstop® 2 -
Description
Negative control for clathrin inhibitor Pitstop® 2 (ab120687) -
Biological description
Negative control for clathrin inhibitor Pitstop® 2 (ab120687) which is of the same chemical class and has a highly related structure to Pitstop® 2. Inhibits amphiphysin binding to the clathrin TD with an IC50 >100 μM and does not block receptor-mediated endocytosis at concentrations of up to 300 μM (unpublished reports).
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General notes
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Pitstop® is a trademark of Freie Universitat Berlin, Newcastle Innovation Ltd. and Children's Medical Research Institute -
Chemical structure
Properties
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Chemical name
5-(4-Bromobenzylidene)-2-(naphthalen-1-ylamino)-1,3-thiazol-4(5H)-one -
Molecular weight
409.30 -
Molecular formula
C20H13BrN2OS -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Chemical Structure - Pitstop® 2 - negative control, Negative control for clathrin inhibitor Pitstop® 2 (ab120688)2D chemical structure image of ab120688, Pitstop® 2 - negative control, Negative control for clathrin inhibitor Pitstop®2
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (14)
ab120688 has been referenced in 14 publications.
- Králová J et al. Sterolight as imaging tool to study sterol uptake, trafficking and efflux in living cells. Sci Rep 12:6264 (2022). PubMed: 35428843
- Abdul-Salam VB et al. CLIC4/Arf6 Pathway. Circ Res 124:52-65 (2019). PubMed: 30582444
- Martínez-Muñoz L et al. Separating Actin-Dependent Chemokine Receptor Nanoclustering from Dimerization Indicates a Role for Clustering in CXCR4 Signaling and Function. Mol Cell 70:106-119.e10 (2018). PubMed: 29625032
- Albecka A et al. HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis. Traffic 17:21-39 (2016). PubMed: 26459807
- Chang HF et al. Endocytosis of Cytotoxic Granules Is Essential for Multiple Killing of Target Cells by T Lymphocytes. J Immunol 197:2473-84 (2016). PubMed: 27527597
