(±)-Blebbistatin, inhibitor of myosin II (ab120425)
Key features and details
- Potent, selective, inhibitor of myosin II
- CAS Number: 674289-55-5
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
(±)-Blebbistatin, inhibitor of myosin II -
Description
Potent, selective, inhibitor of myosin II -
Biological description
Cell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 μM). Inhibits actin-activated MgATPase activity and in vitro motility of class II myosins. Also inhibits contraction of the cytokinetic ring. Blocks cell blebbing and disrupts directed cell migration and cytokinesis.
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CAS Number
674289-55-5 -
Chemical structure
Properties
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Chemical name
(±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one -
Molecular weight
292.34 -
Molecular formula
C18H16N2O2 -
PubChem identifier
3476986 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cc2ccc3N=C1N(CCC1(O)C(=O)c3c2)c4ccccc4 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - (±)-Blebbistatin, inhibitor of myosin II (ab120425)2D chemical structure image of ab120425, Blebbistatin, inhibitor of myosin II
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Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (ab120425)ab32084 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin (ab120425), by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120425 ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32084 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (25)
ab120425 has been referenced in 25 publications.
- Hu S et al. Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-ß-catenin-STAT3 signaling. Cell Death Differ 29:642-656 (2022). PubMed: 34743203
- Lyu Y et al. Beat-to-beat dynamic regulation of intracellular pH in cardiomyocytes. iScience 25:103624 (2022). PubMed: 35005560
- Chang ACY et al. Increased tissue stiffness triggers contractile dysfunction and telomere shortening in dystrophic cardiomyocytes. Stem Cell Reports 16:2169-2181 (2021). PubMed: 34019816
- Gong Z et al. Recursive feedback between matrix dissipation and chemo-mechanical signaling drives oscillatory growth of cancer cell invadopodia. Cell Rep 35:109047 (2021). PubMed: 33909999
- Yang Q et al. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nat Cell Biol 23:733-744 (2021). PubMed: 34155381
