Rhod-2 AM, fluorescent Ca2+ indicator (ab142780)
Key features and details
- Cell-permeable fluorescent Ca2+ indicator
- CAS Number: 145037-81-6
- Purity: > 93%
- Soluble in DMSO to 1 mg/ml
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Rhod-2 AM, fluorescent Ca2+ indicator -
Description
Cell-permeable fluorescent Ca2+ indicator -
Purity
> 93% -
General notes
The Ester Rhod-2 once hydrolyzed by cellular endogenous esterases will bind Ca2+ and localizes to mitochondria,
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CAS Number
145037-81-6 -
Chemical structure
Properties
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Excitation
552nm -
Emission
581nm -
Chemical name
9-[4-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-3,6-bis(dimethylamino)xanthylium bromide -
Molecular weight
1123.96 -
Molecular formula
C52H59BrN4O19 -
PubChem identifier
25229581 -
Storage instructions
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO to 1 mg/ml -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
For more information on AM esters please visit our AM esters FAQ page.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=CC(=C3C4=C(C=C(C=C4)N(C)C)OC5=C3C=CC(=C5)N(C)C)C=CC2=[N+](CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C.[Br-] -
Source
Synthetic
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Research areas
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (20)
ab142780 has been referenced in 20 publications.
- Jiang M et al. Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells. Br J Pharmacol 180:1862-1877 (2023). PubMed: 36740831
- Yao H et al. RCN2 promotes Nasopharyngeal carcinoma progression by curbing Calcium flow and Mitochondrial apoptosis. Cell Oncol (Dordr) 46:1031-1048 (2023). PubMed: 36952101
- Yang J et al. Mitochondria-associated membrane protein PACS2 maintains right cardiac function in hypobaric hypoxia. iScience 26:106328 (2023). PubMed: 36968068
- Pierre M et al. Cardiac involvement in patient-specific induced pluripotent stem cells of myotonic dystrophy type 1: unveiling the impact of voltage-gated sodium channels. Front Physiol 14:1258318 (2023). PubMed: 37791351
- Yu C et al. Chronological adhesive cardiac patch for synchronous mechanophysiological monitoring and electrocoupling therapy. Nat Commun 14:6226 (2023). PubMed: 37803005