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    products/biochemicals/rhod-2-am-fluorescent-ca2-indicator-ab142780.pdf

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Biochemicals Chemical Type Biochemicals
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Rhod-2 AM, fluorescent Ca2+ indicator (ab142780)

  • Datasheet
  • COA
Reviews (3) Submit a question References (10)

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Chemical Structure - Rhod-2 AM, fluorescent Ca<sup>2+</sup> indicator (ab142780)

    Key features and details

    • Cell-permeable fluorescent Ca2+ indicator
    • CAS Number: 145037-81-6
    • Purity: > 93%
    • Soluble in DMSO to 1 mg/ml
    • Form / State: Solid
    • Source: Synthetic

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    Overview

    • Product name

      Rhod-2 AM, fluorescent Ca2+ indicator
    • Description

      Cell-permeable fluorescent Ca2+ indicator
    • Purity

      > 93%
    • General notes

      The Ester Rhod-2 once hydrolyzed by cellular endogenous esterases will bind Ca2+ and localizes to mitochondria,

    • CAS Number

      145037-81-6
    • Chemical structure

      Chemical Structure

    Properties

    • Excitation

      552nm
    • Emission

      581nm
    • Chemical name

      9-[4-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-3,6-bis(dimethylamino)xanthylium bromide
    • Molecular weight

      1123.96
    • Molecular formula

      C52H59BrN4O19
    • PubChem identifier

      25229581
    • Storage instructions

      Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions.
    • Solubility overview

      Soluble in DMSO to 1 mg/ml
    • Handling

      Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

      For more information on AM esters please visit our AM esters FAQ page.

      Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

    • SMILES

      CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=CC(=C3C4=C(C=C(C=C4)N(C)C)OC5=C3C=CC(=C5)N(C)C)C=CC2=[N+](CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C.[Br-]
    • Source

      Synthetic

    • Research areas

      • Biochemicals
      • Chemical Type
      • Biochemicals
      • Biochemicals
      • Chemical Type
      • Fluorescent tools
      • Biochemicals
      • Pharmacology
      • Ion Channels
      • Ionophores, Indicators & Chelators
      • Biochemicals
      • Pharmacology
      • Signaling
      • Ca2+ signaling
      • Ionophores, Indicators & Chelators
      • Biochemicals
      • Pharmacology
      • Signaling
      • Ca2+ signaling
      • Ca2+ binding protein
      • Kits/ Lysates/ Other
      • Tools and Reagents
      • Fluorescent dyes and reagents
      • Biochemicals
      • Chemical Type
      • Dyes, stains and fluorescent tools

    Images

    • Chemical Structure - Rhod-2 AM, fluorescent Ca<sup>2+</sup> indicator (ab142780)
      Chemical Structure - Rhod-2 AM, fluorescent Ca2+ indicator (ab142780)
      2D chemical structure image of ab142780, Rhod-2 AM, fluorescent Ca2+ indicator

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download
    • COA

    References (10)

    Publishing research using ab142780? Please let us know so that we can cite the reference in this datasheet.

    ab142780 has been referenced in 10 publications.

    • Tian F & Zhang Y Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering. Front Cell Dev Biol 9:636553 (2021). PubMed: 33869181
    • Massey N  et al. Organic dust-induced mitochondrial dysfunction could be targeted via cGAS-STING or cytoplasmic NOX-2 inhibition using microglial cells and brain slice culture models. Cell Tissue Res 384:465-486 (2021). PubMed: 33687557
    • Ye L  et al. Carbon Nanotube-Hydrogel Composites Facilitate Neuronal Differentiation While Maintaining Homeostasis of Network Activity. Adv Mater 33:e2102981 (2021). PubMed: 34453367
    • Binning W  et al. Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice. Brain Behav Immun 88:791-801 (2020). PubMed: 32434046
    • Ramachandran RP  et al. Mitochondrial Targeting of the Enteropathogenic Escherichia coli Map Triggers Calcium Mobilization, ADAM10-MAP Kinase Signaling, and Host Cell Apoptosis. mBio 11:N/A (2020). PubMed: 32934081
    • Teo CH  et al. Lithium chloride enhances serotonin induced calcium activity in EGFP-GnIH neurons. Sci Rep 10:13876 (2020). PubMed: 32807874
    • Jegal HG  et al. Ruthenium red improves blastocyst developmental competence by regulating mitochondrial Ca2+ and mitochondrial functions in fertilized porcine oocytes in vitro. J Reprod Dev 66:377-386 (2020). PubMed: 32321875
    • Segel M  et al. Niche stiffness underlies the ageing of central nervous system progenitor cells. Nature 573:130-134 (2019). PubMed: 31413369
    • Sabogal-Guáqueta AM  et al. Linalool attenuates oxidative stress and mitochondrial dysfunction mediated by glutamate and NMDA toxicity. Biomed Pharmacother 118:109295 (2019). PubMed: 31545255
    • Alarcon MML  et al. Cardiac arrhythmias after renal I/R depend on IL-1ß. J Mol Cell Cardiol 131:101-111 (2019). PubMed: 31029578

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Rhod-2 AM used for Live imaging

    Good
    Abreviews
    Abreviews
    abreview image
    Rhod-2 AM was used for Live imaging in HeLa cells. It is used at a concentration of 1uM in HBSS for 15 min at 37 degrees.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 25 2019

    Calcium Staining

    Excellent
    Abreviews
    Abreviews
    abreview image
    SH-SY5Y cells were grown overnight on coverslips treated with histogrip. Rhod-2AM was prepared in serum free DMEM-F12 media to a final concentration of 5 μm and used to treat the cells for 30 minutes. Negative controls were treated with serum free DMEM-F12 media without Rhod-2AM. Following treatment, cells were washed gently in PBS 3 times and fixed with 4% PFA for 10 minutes. Cells were washed and stained with DAPI for 2 minutes, and mounted on slides using Antifade fluorescence mounting media.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 28 2018

    Rhod-2AM Calcium Staining

    Excellent
    Abreviews
    Abreviews
    SH-SY5Y cells were grown overnight on coverslips treated with histogrip. Rhod-2AM was prepared in serum free DMEM-F12 media to a final concentration of 5 μm and used to treat the cells for 30 minutes. Negative controls were treated with serum free DMEM-F12 media without Rhod-2AM. Following treatment, cells were washed gently in PBS 3 times and fixed with 4% PFA for 10 minutes. Cells were then stained with DAPI for 2 minutes, and mounted on slides using Antifade fluorescence mounting media.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Anu Antony

    Verified customer

    Submitted Feb 27 2018

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