(R,S)-CHPG, mGlu5 agonist (ab120039)
Key features and details
- Selective mGlu5 agonist
- CAS Number: 170846-74-9
- Soluble in 1 eq. NaOH to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
(R,S)-CHPG, mGlu5 agonist -
Description
Selective mGlu5 agonist -
Biological description
Selective mGlu5 receptor agonist. Water soluble form available - see (ab120221)
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CAS Number
170846-74-9 -
Chemical structure
Properties
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Chemical name
(R,S)-2-Amino-2-(2-chloro-5-hydroxyphenyl)acetic acid -
Molecular weight
201.61 -
Molecular formula
C8H8NO3Cl -
PubChem identifier
3645780 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in 1 eq. NaOH to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NC(c1cc(O)ccc1Cl)C(=O)O -
Source
Synthetic
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Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - (R,S)-CHPG, mGlu5 agonist (ab120039)2D chemical structure image of ab120039, (R,S)-CHPG, mGlu5 agonist
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Functional Studies - (R,S)-CHPG, mGlu5 agonist (ab120039)SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (R,S)-CHPG (ab120039). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase in (R,S)-CHPG concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (3)
ab120039 has been referenced in 3 publications.
- Fontanez-Nuin DE et al. Memory for fear extinction requires mGluR5-mediated activation of infralimbic neurons. Cereb Cortex 21:727-35 (2011). PubMed: 20705895
- Le Duigou C & Kullmann DM Group I mGluR agonist-evoked long-term potentiation in hippocampal oriens interneurons. J Neurosci 31:5777-81 (2011). PubMed: 21490219
- Besheer J et al. Interoceptive effects of alcohol require mGlu5 receptor activity in the nucleus accumbens. J Neurosci 29:9582-91 (2009). PubMed: 19641121