(R,S)-MCPG, Group I /II metabotropic glutamate antagonist (ab120033)
Key features and details
- Group I /II metabotropic glutamate antagonist
- CAS Number: 146669-29-6
- Soluble in 1 eq. NaOH to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
(R,S)-MCPG, Group I /II metabotropic glutamate antagonist -
Description
Group I /II metabotropic glutamate antagonist -
Biological description
Group I / group II metabotropic glutamate receptor antagonist.
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CAS Number
146669-29-6 -
Chemical structure
Properties
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Chemical name
(R,S)-α-Methyl-4-carboxyphenylglycine -
Molecular weight
209.20 -
Molecular formula
C10H11NO4 -
PubChem identifier
1222 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in 1 eq. NaOH to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NC(C)(C(=O)O)c1ccc(cc1)C(=O)O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - (R,S)-MCPG, Group I /II metabotropic glutamate antagonist (ab120033)2D chemical structure image of ab120033, (R,S)-MCPG, Group I /II metabotropic glutamate antagonist
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Immunocytochemistry/ Immunofluorescence - (R,S)-MCPG, Group I /II metabotropic glutamate antagonist (ab120033)ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-MCPG (ab120033), by ICC/IF. Decrease in FMRP expression correlates with increased concentration of (R,S)-MCPG, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120033 ((R,S)-MCPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (7)
ab120033 has been referenced in 7 publications.
- Kosillo P et al. Cortical Control of Striatal Dopamine Transmission via Striatal Cholinergic Interneurons. Cereb Cortex N/A:N/A (2016). PubMed: 27566978
- Hedrick T & Waters J Acetylcholine excites neocortical pyramidal neurons via nicotinic receptors. J Neurophysiol 113:2195-209 (2015). PubMed: 25589590
- Chamma I et al. Activity-dependent regulation of the K/Cl transporter KCC2 membrane diffusion, clustering, and function in hippocampal neurons. J Neurosci 33:15488-503 (2013). PubMed: 24068817
- Locatelli F et al. Late-onset bursts evoked by mossy fibre bundle stimulation in unipolar brush cells: evidence for the involvement of H- and TRP-currents. J Physiol 591:899-918 (2013). PubMed: 23129798
- Bouthour W et al. A human mutation in Gabrg2 associated with generalized epilepsy alters the membrane dynamics of GABAA receptors. Cereb Cortex 22:1542-53 (2012). PubMed: 21908847
- Jo YH Endogenous BDNF regulates inhibitory synaptic transmission in the ventromedial nucleus of the hypothalamus. J Neurophysiol 107:42-9 (2012). PubMed: 21994261
- Liotta A et al. Energy demand of synaptic transmission at the hippocampal Schaffer-collateral synapse. J Cereb Blood Flow Metab 32:2076-83 (2012). PubMed: 22929439
