(R,S)-Rolipram, Selective PDE4 inhibitor (ab120029)
Key features and details
- Selective PDE4 inhibitor
- CAS Number: 61413-54-5
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
(R,S)-Rolipram, Selective PDE4 inhibitor -
Description
Selective PDE4 inhibitor -
Biological description
Selective cAMP-specific phosphodiesterase PDE4 inhibitor (IC50 values are 1.1 and 0.17 μM for inhibition of mouse PDE4A1 and PDE4B, respectively)
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CAS Number
61413-54-5 -
Chemical structure
Properties
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Chemical name
(R,S)-4-(3-(Cyclopentyloxy)-4-methoxyphenyl)pyrrolidin-2-one -
Molecular weight
275.35 -
Molecular formula
C16H21NO3 -
PubChem identifier
5092 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COc2ccc(cc2OC1CCCC1)C3CC(=O)NC3 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - (R,S)-Rolipram, Selective PDE4 inhibitor (ab120029)2D chemical structure image of ab120029, (R,S)-Rolipram, Selective PDE4 inhibitor
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Immunocytochemistry/ Immunofluorescence - (R,S)-Rolipram, Selective PDE4 inhibitor (ab120029)ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (6)
ab120029 has been referenced in 6 publications.
- Nelissen E et al. The sGC stimulator BAY-747 and activator runcaciguat can enhance memory in vivo via differential hippocampal plasticity mechanisms. Sci Rep 12:3589 (2022). PubMed: 35246566
- Severino J et al. Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cells. EMBO J 41:e109457 (2022). PubMed: 35603814
- Ahmad F et al. Chemical Stimulation of Rodent and Human Cortical Synaptosomes: Implications in Neurodegeneration. Cells 10:N/A (2021). PubMed: 34065927
- Nelissen E et al. Soluble Guanylate Cyclase Stimulator Vericiguat Enhances Long-Term Memory in Rats without Altering Cerebral Blood Volume. Biomedicines 9:N/A (2021). PubMed: 34440254
- Murase Y et al. Long-term expansion with germline potential of human primordial germ cell-like cells in vitro. EMBO J 39:e104929 (2020). PubMed: 32954504
- Shelly M et al. Local and long-range reciprocal regulation of cAMP and cGMP in axon/dendrite formation. Science 327:547-52 (2010). PubMed: 20110498