(S)-AMPA, agonist (ab120005)
Key features and details
- AMPA agonist
- CAS Number: 83643-88-3
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
(S)-AMPA, agonist -
Description
AMPA agonist -
Alternative names
- GLUR 1
-
CAS Number
83643-88-3 -
Chemical structure
Properties
-
Chemical name
(S)-α-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid -
Molecular weight
186.17 -
Molecular formula
C7H10N2O4 -
PubChem identifier
158397 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
N[C@@H](Cc1c(C)onc1O)C(=O)O -
Source
Synthetic
-
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
Images
-
Chemical Structure - (S)-AMPA, agonist (ab120005)2D chemical structure image of ab120005, (S)-AMPA, agonist
-
Functional Studies - (S)-AMPA, agonist (ab120005)Sims et al PLoS One. 2013;8(1):e53814. doi: 10.1371/journal.pone.0053814. Epub 2013 Jan 11. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Release of adenosine by depolarisation and agonists.
(Panel d) Adenosine released was evoked after depolarisation with AMPA.
-
Functional Studies - (S)-AMPA, agonist (ab120005)ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-AMPA (ab120005), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-AMPA, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120005 ((S)-AMPA) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (14)
ab120005 has been referenced in 14 publications.
- Awasthi A et al. Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting. Science 363:N/A (2019). PubMed: 30545844
- Sebe JY et al. Ca2+-Permeable AMPARs Mediate Glutamatergic Transmission and Excitotoxic Damage at the Hair Cell Ribbon Synapse. J Neurosci 37:6162-6175 (2017). PubMed: 28539424
- Winland CD et al. Inflammation alters AMPA-stimulated calcium responses in dorsal striatal D2 but not D1 spiny projection neurons. Eur J Neurosci 46:2519-2533 (2017). PubMed: 28921719
- Brandalise F & Gerber U Mossy fiber-evoked subthreshold responses induce timing-dependent plasticity at hippocampal CA3 recurrent synapses. Proc Natl Acad Sci U S A : (2014). PubMed: 24550458
- Sims RE et al. Sleep-wake sensitive mechanisms of adenosine release in the basal forebrain of rodents: an in vitro study. PLoS One 8:e53814 (2013). PubMed: 23326515
