SN 38, DNA topoisomerase I inhibitor (ab141108)
Key features and details
- DNA topoisomerase I inhibitor. Active metabolite of CPT-11.
- CAS Number: 86639-52-3
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
SN 38, DNA topoisomerase I inhibitor -
Description
DNA topoisomerase I inhibitor. Active metabolite of CPT-11. -
Alternative names
- 7-Ethyl-10-hydroxycamptothecin
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Biological description
Active metabolite of CPT-11 (Irinotecan) (ab141107). Inhibits DNA topoisomerase I (IC50 values are 0.74 and 1.9 μM in P388 and Ehrlich cells respectively) and DNA and RNA synthesis (IC50 values are 77 nM and 1.3 μM respectively). Shows potent antitumor activity, causes S- and G2 cell cycle arrest and induces apoptosis. Active in vivo.
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Purity
> 99% -
CAS Number
86639-52-3 -
Chemical structure
Properties
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Chemical name
(4S)-4,11-Diethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione -
Molecular weight
392.41 -
Molecular formula
C22H20N2O5 -
PubChem identifier
104842 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC[C@]1(O)C=2C=C3c4nc5ccc(O)cc5c(CC)c4CN3C(=O)C=2COC1=O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - SN 38, DNA topoisomerase I inhibitor (ab141108)2D chemical structure image of ab141108, SN 38, DNA topoisomerase I inhibitor
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Immunocytochemistry/ Immunofluorescence - SN 38, DNA topoisomerase I inhibitor (ab141108)ab2893 staining γH2A.X in HeLa cells treated with SN 38 (ab141108), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of SN 38, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141108 (SN 38) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab141108 has been referenced in 2 publications.
- Winkler C et al. SLFN11 informs on standard of care and novel treatments in a wide range of cancer models. Br J Cancer 124:951-962 (2021). PubMed: 33339894
- Peter S et al. Cell-based high-throughput compound screening reveals functional interaction between oncofetal HMGA2 and topoisomerase I. Nucleic Acids Res N/A:N/A (2016). PubMed: 27587582