Stattic, STAT3 inhibitor (ab120952)
Key features and details
- Selective STAT3 inhibitor
- CAS Number: 19983-44-9
- Purity: > 99%
- Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Stattic, STAT3 inhibitor -
Description
Selective STAT3 inhibitor -
Purity
> 99% -
CAS Number
19983-44-9 -
Chemical structure
Properties
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Chemical name
6-Nitrobenzo[b]thiophene-1,1-dioxide -
Molecular weight
211.19 -
Molecular formula
C8H5NO4S -
PubChem identifier
2779853 -
Storage instructions
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[O-][N+](=O)c1ccc2C=CS(=O)(=O)c2c1 -
Source
Synthetic
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Research areas
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- JAK/STAT signaling
- STAT
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- JAK/STAT signaling
- STAT
- Inhibitors
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Stattic, STAT3 inhibitor (ab120952)2D chemical structure image of ab120952, Stattic, STAT3 inhibitor
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Functional Studies - Stattic, STAT3 inhibitor (ab120952)ab76315 staining STAT3 (phospho Y705) in MDA-MB-231 cells treated with stattic (ab120952), by ICC/IF. Decrease in STAT3 (phospho Y705) expression correlates with increased concentration of stattic as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120952 (stattic) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76315 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (23)
ab120952 has been referenced in 23 publications.
- Miyamoto T et al. B7-H3 Suppresses Antitumor Immunity via the CCL2-CCR2-M2 Macrophage Axis and Contributes to Ovarian Cancer Progression. Cancer Immunol Res 10:56-69 (2022). PubMed: 34799346
- Mantsounga CS et al. Macrophage IL-1ß promotes arteriogenesis by autocrine STAT3- and NF-?B-mediated transcription of pro-angiogenic VEGF-A. Cell Rep 38:110309 (2022). PubMed: 35108537
- Liu Q et al. CCL5 Suppresses Klotho Expression via p-STAT3/DNA Methyltransferase1-Mediated Promoter Hypermethylation. Front Physiol 13:856088 (2022). PubMed: 35299661
- Timmons GA et al. The Circadian Clock Protein BMAL1 Acts as a Metabolic Sensor In Macrophages to Control the Production of Pro IL-1ß. Front Immunol 12:700431 (2021). PubMed: 34858390
- Liu Z et al. Ubiquitin-Specific Protease 25 Aggravates Acute Pancreatitis and Acute Pancreatitis-Related Multiple Organ Injury by Destroying Tight Junctions Through Activation of The STAT3 Pathway. Front Cell Dev Biol 9:806850 (2021). PubMed: 35096833