Thapsigargin, Ca2+-ATPase inhibitor (ab120286)
Key features and details
- Ca2+-ATPase inhibitor
- CAS Number: 67526-95-8
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Thapsia garganica
Overview
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Product name
Thapsigargin, Ca2+-ATPase inhibitor -
Description
Ca2+-ATPase inhibitor -
Biological description
A potent, cell-permeable Ca2+-ATPase inhibitor. Releases Ca2+ by inhibiting endoplasmic reticular Ca2+-ATPase (IC50 = 4-13 nM). Both tumorogenic and apoptotic actions reported.
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CAS Number
67526-95-8 -
Chemical structure
Properties
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Chemical name
(3S,3aR,4S,6S,6aR,7S,8S,9bS)-6-(Acetyloxy)-2,3,3a,4,5,6,6a,7,8,9b-decahydro-3,3a-dihydroxy-3,6,9-trimethyl-8-[[(2Z)-2-methyl-1-oxo-2-butenyl]oxy]-2-oxo-4-(1-oxobutoxy)azuleno[4,5-b]furan-7-yl octanoate -
Molecular weight
650.76 -
Molecular formula
C34H50O12 -
PubChem identifier
446378 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC=C(C)C(=O)O[C@@H]3[C@@H](OC(=O)CCCCCCC)[C@H]1C([C@@H]2OC(=O)[C@](O)(C)[C@@]2(O)[C@H](C[C@]1(C)OC(C)=O)OC(=O)CCC)=C3C -
Source
Thapsia garganica
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Thapsigargin, Ca2+-ATPase inhibitor (ab120286)2D chemical structure image of ab120286, Thapsigargin, Ca2+-ATPase inhibitor
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Functional Studies - Thapsigargin, Ca2+-ATPase inhibitor (ab120286)ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin (ab120286), by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286 (thapsigargin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (31)
ab120286 has been referenced in 31 publications.
- Qiu K et al. Ryanodine receptor RyR1-mediated elevation of Ca2+ concentration is required for the late stage of myogenic differentiation and fusion. J Anim Sci Biotechnol 13:9 (2022). PubMed: 35144690
- Zhang P et al. Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma. Signal Transduct Target Ther 7:46 (2022). PubMed: 35169126
- Ye Z et al. Arachidonic acid-regulated calcium signaling in T cells from patients with rheumatoid arthritis promotes synovial inflammation. Nat Commun 12:907 (2021). PubMed: 33568645
- Corman A et al. A chemical screen for modulators of mRNA translation identifies a distinct mechanism of toxicity for sphingosine kinase inhibitors. PLoS Biol 19:e3001263 (2021). PubMed: 34033645
- Gupta K et al. Bile canaliculi contract autonomously by releasing calcium into hepatocytes via mechanosensitive calcium channel. Biomaterials 259:120283 (2020). PubMed: 32827796