trans-Retinoic acid, Stem cell differentiator (ab120728)
Key features and details
- Vitamin A metabolite. Stem cell differentiator.
- CAS Number: 302-79-4
- Purity: > 99%
- Soluble in DMSO to 25 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
trans-Retinoic acid, Stem cell differentiator -
Description
Vitamin A metabolite. Stem cell differentiator. -
Alternative names
- ATRA
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Purity
> 99% -
CAS Number
302-79-4 -
Chemical structure
Properties
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Chemical name
3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2E,4E,6E,8E-nonatetraenoic acid -
Molecular weight
300.44 -
Molecular formula
C20H28O2 -
Storage instructions
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 25 mM and in ethanol to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - trans-Retinoic acid, Stem cell differentiator (ab120728)2D chemical structure image of ab120728, trans-Retinoic acid, Stem cell differentiator
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Immunocytochemistry/ Immunofluorescence - trans-Retinoic acid, Stem cell differentiator (ab120728)ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid (ab120728), by ICC/IF. Decrease of Oct4 expression correlates with increased concentration of trans-retinoic acid, as described in literature.
The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19857 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (3)
ab120728 has been referenced in 3 publications.
- Dolce LG et al. Myosin Va interacts with the exosomal protein spermine synthase. Biosci Rep 39:N/A (2019). PubMed: 30733278
- Maina MB et al. The involvement of tau in nucleolar transcription and the stress response. Acta Neuropathol Commun 6:70 (2018). PubMed: 30064522
- Zhou TB et al. Effect of all-trans retinoic acid treatment on prohibitin and renin-angiotensin-aldosterone system expression in hypoxia-induced renal tubular epithelial cell injury. J Renin Angiotensin Aldosterone Syst 15:243-9 (2014). PubMed: 25031298