Triptolide from Tripterygium wilfordii, Anti-inflammatory agent (ab120720)
Key features and details
- Anti-inflammatory agent with multiple biological activities
- CAS Number: 38748-32-2
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Tripterygium wilfordii
Overview
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Product name
Triptolide from Tripterygium wilfordii, Anti-inflammatory agent -
Description
Anti-inflammatory agent with multiple biological activities -
Alternative names
- PG490
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Biological description
Active ingredient of the vineTripterygium wilfodii Hook. f. Anti-inflammatory and immunosuppressive agent. Inhibits NF-κB transcriptional activity. Additionally, shows antiproliferative and pro-apoptotic activites. Downregulates RAC1, JAK/STAT 3 pathway and has anticancer properties.
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Purity
> 99% -
CAS Number
38748-32-2 -
Chemical structure
Properties
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Chemical name
(3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)-3b,4,4a,6,6a,7a,7b,8b,9,10-Decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)trisoxireno[4b,5:6,7:8a,9]phenanthro[1,2-c]furan-1(3H)-one -
Molecular weight
360.41 -
Molecular formula
C20H24O6 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Tripterygium wilfordii
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Triptolide from Tripterygium wilfordii, Anti-inflammatory agent (ab120720)2D chemical structure image of ab120720, Triptolide from Tripterygium wilfordii, Anti-inflammatory agent
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Immunocytochemistry/ Immunofluorescence - Triptolide from Tripterygium wilfordii, Anti-inflammatory agent (ab120720)ab22758 staining nucleolin in HeLa cells treated with Triptolide from Tripterygium wilfordii (ab120720), by ICC/IF. Changes in nuclear localization of nucleolin correlates with increased concentration of Triptolide from Tripterygium wilfordii, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120720 (Triptolide from Tripterygium wilfordii ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab22758 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab120720 has been referenced in 2 publications.
- Gillen SL et al. Differential regulation of mRNA fate by the human Ccr4-Not complex is driven by coding sequence composition and mRNA localization. Genome Biol 22:284 (2021). PubMed: 34615539
- Zhang H et al. DEAD-Box Helicase 18 Counteracts PRC2 to Safeguard Ribosomal DNA in Pluripotency Regulation. Cell Rep 30:81-97.e7 (2020). PubMed: 31914400