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    products/biochemicals/tunicamycin-protein-glycosylation-inhibitor-ab120296.pdf

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Microbiology Antimicrobial Antibiotic
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Tunicamycin, Protein glycosylation inhibitor (ab120296)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (1)References (17)

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Chemical Structure - Tunicamycin, Protein glycosylation inhibitor (ab120296)
  • Western blot - Tunicamycin, Protein glycosylation inhibitor. (ab120296)
  • Immunocytochemistry - Tunicamycin, Protein glycosylation inhibitor (ab120296)

Key features and details

  • Antibiotic. Protein glycosylation inhibitor.
  • CAS Number: 11089-65-9
  • Soluble in DMSO to 50 mM
  • Form / State: Solid
  • Source: Streptomyces sp.

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Overview

  • Product name

    Tunicamycin, Protein glycosylation inhibitor
  • Description

    Antibiotic. Protein glycosylation inhibitor.
  • Biological description

    Nucleoside antibiotic that inhibits protein glycosylation. Inhibits GlcNAc phosphotransferase (GPT) and inhibits the transfer of N-acetylglucosamine-1-phosphate from UDP-N-acetylglucosamine to dolichol phosphate in the first step of glycoprotein synthesis. Inhibits N-linked glycosylation and blocks the formation of N-glycosidic protein-carbohydrate linkages. Active in vitro against Gram-positive bacteria, yeasts, fungi and viruses.

  • General notes

    This compound is a mixture of the homologs of tunicamycin: A, B, C and D which vary in the carbon chain length (n=8 to 11)

  • CAS Number

    11089-65-9
  • Chemical structure

    Chemical Structure

Properties

  • Molecular weight

    844.95
  • Molecular formula

    C39H64N4O16 (for tunicamycin C, n = 10)
  • PubChem identifier

    16220051
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 50 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Toxic, refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(C)CCCCCCCC/C=C/C(=O)N[C@@H]1[C@H]([C@H]([C@H](O[C@H]1O[C@H]2[C@H]([C@@H]([C@H]([C@@H](O2)CO)O)O)NC(=O)C)C[C@H]([C@@H]3[C@H]([C@H]([C@@H](O3)N4C=CC(=O)NC4=O)O)O)O)O)O
  • Source

    Streptomyces sp.

  • Research areas

    • Microbiology
    • Antimicrobial
    • Antibiotic
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Chemical Type
    • Antibiotic
    • Biochemicals
    • Pharmacology
    • Enzymes
    • Transferase
    • General
    • Biochemicals
    • Pharmacology
    • Signaling
    • Immunomodulators
    • Antibacterial

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Tunicamycin, Protein glycosylation inhibitor (ab120296)
    Chemical Structure - Tunicamycin, Protein glycosylation inhibitor (ab120296)
    2D chemical structure image of ab120296, Tunicamycin, Protein glycosylation inhibitor
  • Western blot - Tunicamycin, Protein glycosylation inhibitor. (ab120296)
    Western blot - Tunicamycin, Protein glycosylation inhibitor. (ab120296)
    All lanes : Anti-ATF-4 antibody [EPR18111] (ab184909) at 1/1000 dilution

    Lane 1 : Untreated HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (control)
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) treated with 5 µM MG-132 (ab141003) and 3 µg/ml tunicamycin (ab120296) for 6 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22095285).

  • Immunocytochemistry - Tunicamycin, Protein glycosylation inhibitor (ab120296)
    Immunocytochemistry - Tunicamycin, Protein glycosylation inhibitor (ab120296)

    ab11419 staining DDIT3 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells +/- Tunicamycin 1.5μM, 6 hours (ab120296). 

    The cells were fixed with 4% PFA (10 min), permeabilized with 0.1% Triton-X for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab11419 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (17)

Publishing research using ab120296? Please let us know so that we can cite the reference in this datasheet.

ab120296 has been referenced in 17 publications.

  • Hu S  et al. Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-ß-catenin-STAT3 signaling. Cell Death Differ 29:642-656 (2022). PubMed: 34743203
  • Wang D  et al. Upregulation of C/EBP Homologous Protein induced by ER Stress Mediates Epithelial to Myofibroblast Transformation in ADTKD-UMOD. Int J Med Sci 19:364-376 (2022). PubMed: 35165522
  • Wang Y  et al. Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy. Cell Biosci 11:82 (2021). PubMed: 33933165
  • Konovalova J  et al. Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs. Int J Mol Sci 22:N/A (2021). PubMed: 34575854
  • Mahameed M  et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun 11:1304 (2020). PubMed: 32161259
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Has used two vials of Tunicamycin from another company that would not go into solution in DMSO. Tried heating, agitation overnight. Was told that it will dissolve in hot methanol but might not be stable after doing this.
What is the procedure that is used to solubilize ab120296 in DMSOto 50 mM? Is it stable after solubilization?

Read More

Abcam community

Verified customer

Asked on Aug 13 2012

Answer

Thank you very much for your call last week and for your patience while I have been in touch with our biochemical scientists regarding your enquiry.

We quote solubility in DMSO of 50mM and believe it's quite easily dissolved at room temperature – no special procedures have been recorded. We also run the NMR in DMSO so we are confident that there won't be an issue.

We do fully guarantee ab120296 to work as stated on the datasheet, and if you have any problems at all with it we will be happy to refund or replace it for you.
I hope that this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on Aug 13 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

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