Z-IETD-FMK, Caspase-8 inhibitor (ab141382)
Key features and details
- Caspase-8 inhibitor
- CAS Number:
- Purity: > 99%
- Form / State: Lyophilized
- Source: Synthetic
Overview
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Product name
Z-IETD-FMK, Caspase-8 inhibitor -
Description
Caspase-8 inhibitor -
Purity
> 99% -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 1148 IETD-FMK, Z-,. 1148-5 is the same size as the 5 mg size of ab141382.
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Chemical structure
Properties
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Molecular weight
654.70 -
Molecular formula
C30H43FN4O11 -
Sequence
IETD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Glu-2 = Glu(OMe); Asp-4 = Asp(OMe)) -
PubChem identifier
44135212 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CCC(C)C(C(=O)NC(CCC(=O)OC)C(=O)NC(C(C)O)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O) OCC1=CC=CC=C1 -
Source
Synthetic
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Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Z-IETD-FMK, Caspase-8 inhibitor (ab141382)2D chemical structure image of ab141382, Z-IETD-FMK, Caspase-8 inhibitor
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Functional Studies - Z-IETD-FMK, Caspase-8 inhibitor (ab141382)ab13847 staining active caspase 3 in SKNSH cells treated with Z-IETD-FMK (ab141382), by ICC/IF. Decrease in active caspase 3 expression correlates with increased concentration of Z-IETD-FMK, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141382 (Z-IETD-FMK) in DMSO. After this incupation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24 hours. The samples were then fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DA
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab141382 has been referenced in 6 publications.
- Musarra-Pizzo M et al. Direct cleavage of caspase-8 by herpes simplex virus 1 tegument protein US11. Sci Rep 12:12317 (2022). PubMed: 35853963
- Yang R et al. SARS-CoV-2 Accessory Protein ORF7b Mediates Tumor Necrosis Factor-a-Induced Apoptosis in Cells. Front Microbiol 12:654709 (2021). PubMed: 34484133
- Wang J et al. Fundc1-dependent mitophagy is obligatory to ischemic preconditioning-conferred renoprotection in ischemic AKI via suppression of Drp1-mediated mitochondrial fission. Redox Biol 30:101415 (2020). PubMed: 31901590
- Yang H et al. Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways. World J Gastrointest Oncol 11:377-392 (2019). PubMed: 31139308
- Liu G et al. Beclin-1-mediated Autophagy Protects Against Cadmium-activated Apoptosis via the Fas/FasL Pathway in Primary Rat Proximal Tubular Cell Culture. Sci Rep 7:977 (2017). PubMed: 28428545
- Zhao Y et al. Berbamine inhibited the growth of prostate cancer cells in vivo and in vitro via triggering intrinsic pathway of apoptosis. Prostate Cancer Prostatic Dis 19:358-366 (2016). PubMed: 27431500