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  1. Link

    products/cell-lysates/e-coli-positive-control-escherichia-coli--whole-cell-lysate-ab5395.pdf

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E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (3)

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Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

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Overview

  • Product name

    E. coli Positive Control (Escherichia coli ) Whole Cell Lysate
  • General notes

    This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:

    GST

    T7

    HSV

    C-Myc

    VSV-g

    Glu-Glu

    V5

    E-tag

    FLAG (DDDDK)

    S-tag

    HA

    6x His

    Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    1X Loading Buffer (1X SDS-PAGE Sample Buffer: 62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue)
  • Concentration information loading...
  • Lysate notes

    Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.

  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Glu-Glu Tag
    • Tags & Cell Markers
    • Epitope Tags
    • V5 Tag
    • Tags & Cell Markers
    • Epitope Tags
    • T7 Tag
    • Microbiology
    • Organism
    • Bacteria
    • Gram negative Bacteria
    • Escherichia coli
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • E tag
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • VSV-G Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • S Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • HSV Tag
    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Arabidopsis / Miscellaneous
  • Background

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

Notes
WB
Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

Images

  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    All lanes : Anti-6X His tag® antibody (ab9108) at 1/1000 dilution

    Lane 1 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
    Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
    Lane 4 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl
  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

    This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab5395? Please let us know so that we can cite the reference in this datasheet.

ab5395 has been referenced in 3 publications.

  • Shokri L  et al. A Comprehensive Drosophila melanogaster Transcription Factor Interactome. Cell Rep 27:955-970.e7 (2019). PubMed: 30995488
  • Májek P  et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). PubMed: 24278455
  • Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . PubMed: 21515633

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

Hey,

Is the concentration of the tagged fusion protein known? If not, do you sell any myc-tagged fusion proteins where the concentration is known?

Read More

Abcam community

Verified customer

Asked on Mar 07 2013

Answer

Thank you for contacting us.
We do not have an exact concentration for this material and do not have another lysate where the exact concentration of the myc-tagged protein is known.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 07 2013

Question

J’aurais besoin de savoir quelle est la souche d’E.Coli utilisée pour le lysat ab5395 (concernant le lysat ab2431 la souche utilisé est BL21).

Merci beaucoup pour votre retour

Cordialement

Read More

Abcam community

Verified customer

Asked on May 17 2012

Answer

J'espère que vous allez bien!

Je vous écrit parce que j'ai finalement reçu les informations par rapportà la souche du lysat ab5395. Le laboratoire m'a confirmé que c'est la souche BL21. Je suis très désolée pour le retard de cette réponse. J'espère que cette information vous est quand-même utile.

Aussi, est-ce que vous avez eu des bons résultats avec le nouveau tube de ab2431?

Encore une fois toutes mes excuses pour ce délais de réponse. N'hésitez pas à nous recontacter si vous avez des autres questions.

Read More

Abcam Scientific Support

Answered on May 17 2012

Question

Do you have the sequence of each of the tags contained in the recombinant protein?

Read More

Abcam community

Verified customer

Asked on Mar 06 2006

Answer

Thank you for your enquiry. Here is the sequence information I have for this product: N' GST - T7 - HSV - C-MYC - VSV-g - GluGlu - a tubulin - V5 - E-tag - Flag - S-tag - HA - 6xHIS C' T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC HA = YPYDVPDYAC VSV-g = YTDIEMNRLGK GluGlu = EYMPME E-tag = GAPVPYPDPLEPR FLAG = DYKDDDDK V5 = TSTLPVDTTDWIEGETYK 6xHIS = HHHHHH I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Read More

Abcam Scientific Support

Answered on Mar 06 2006

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