E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
Overview
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Product name
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate -
General notes
This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
GST
T7
HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
1X Loading Buffer (1X SDS-PAGE Sample Buffer: 62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue) -
Concentration information loading... -
Lysate notes
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
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Research areas
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Background
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
Use 0.5 - 2.0 µl for 10 - 12% gels. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Use 0.5 - 2.0 µl for 10 - 12% gels. |
Images
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Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)All lanes : Anti-6X His tag® antibody (ab9108) at 1/1000 dilution
Lane 1 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
Lane 2 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
Lane 3 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
Lane 4 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl -
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab5395 has been referenced in 3 publications.
- Shokri L et al. A Comprehensive Drosophila melanogaster Transcription Factor Interactome. Cell Rep 27:955-970.e7 (2019). PubMed: 30995488
- Májek P et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). PubMed: 24278455
- Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . PubMed: 21515633