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  1. Link

    products/cell-lysates/molt-4-whole-cell-lysate-ab7912.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Human Other
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MOLT-4 whole cell lysate (ab7912)

  • Datasheet
  • SDS
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Overview

  • Product name

    MOLT-4 whole cell lysate
  • General notes

    Cell line: MOLT4 (Human acute lymphoblastic leukemia).
    Growth media: RPMI and 10% FBS.

     

    MOLT4 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 100% SDS Sample Buffer
  • Concentration information loading...
  • Lysate notes

    MOLT4 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Human
    • Other
  • Background

    MOLT4 cells are lymphoblast-like in morphology and are used for studies of apoptosis, tumor cytotoxicity, tumorigenicity, as well as for antitumour testing.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
MOLT4 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
Notes
WB
Use at an assay dependent concentration.

MOLT4 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab7912? Please let us know so that we can cite the reference in this datasheet.

ab7912 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

vielen Dank für Ihre schnelle Antwort. Wir werden den von Ihnen empfohlenen Isotyp bestellen.
Im Rahmen der Antikörperbestellung möchten wir auch eine Positivkontrolle für den Westernblot (in Form eines Lysats) mitbestellen.
Bei dem IL-9 AK (ab111915) ist auf dem Datasheet angegeben, dass „human fetal liver lysate“ für den Westernblot verwendet wurde. Wie viel µg wurden hier aufgetragen?
Für den IL-9R AK (ab106287) würden wir voraussichtlich „MOLT4 whole cell lysate“ im Westernblot verwenden. Bei einem ausschließlich für Westernblot getesteten IL-9 AK (ab66106) haben Sie dies im Westernblot eingesetzt. Wie viel µg wurden hier auftragen?
Vielen Dank im Voraus.
Mit freundlichen Grüßen

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Abcam community

Verified customer

Asked on Apr 26 2012

Answer

Vielen Dank für Ihre Anfrage.

Für ab111915 wurden 20 µg Lysat aufgetragen, für ab66106 waren es10 µg (siehe Datenblatt). Normalerweise sind - je nach Expressionslevel in der Positivkontrolle - 20 bis maximal 50 µg mehr als ausreichend, bei rekombinantem Protein sogar noch weniger.

Wie Sie vielleicht schon gesehen haben, haben wir die entsprechenden Lysate im Katalog:

ab29890: Liver (Human) Tissue Lysate - fetal normal tissue

https://www.abcam.com/Liver-Human-Tissue-Lysate-fetal-normal-tissue-ab29890.html (or use the following: https://www.abcam.com/Liver-Human-Tissue-Lysate-fetal-normal-tissue-ab29890.html).

ab7912: MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

https://www.abcam.com/MOLT4-whole-cell-lysate-ab7912.html (or use the following: https://www.abcam.com/MOLT4-whole-cell-lysate-ab7912.html).

ab30124: Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor cell line

https://www.abcam.com/Raji-whole-cell-lysate-ab30124.html (or use the following: https://www.abcam.com/Raji-whole-cell-lysate-ab30124.html).

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Abcam Scientific Support

Answered on Apr 26 2012

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