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  1. Link

    products/cell-lysates/raw-2647-whole-cell-lysate-ab7187.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Mouse Other
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RAW 264.7 whole cell lysate (ab7187)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (8)

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Overview

  • Product name

    RAW 264.7 whole cell lysate
  • General notes

    Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor).
    Growth media: DMEM and 10% fetal bovine serum.

     

    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 100% SDS Sample Buffer
  • Concentration information loading...
  • Lysate notes

    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Mouse
    • Other
  • Background

    Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution. Ready to load.
Notes
WB
Use at an assay dependent dilution. Ready to load.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab7187? Please let us know so that we can cite the reference in this datasheet.

ab7187 has been referenced in 8 publications.

  • Manian KV  et al. 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration. Cell Stem Cell 28:846-862.e8 (2021). PubMed: 33784497
  • d'Uscio LV & Katusic ZS Endothelium-specific deletion of amyloid-β precursor protein exacerbates endothelial dysfunction induced by aging. Aging (Albany NY) 13:19165-19185 (2021). PubMed: 34382945
  • Magalhaes RS  et al. A tissue-engineered uterus supports live births in rabbits. Nat Biotechnol 38:1280-1287 (2020). PubMed: 32601434
  • d'Uscio LV & Katusic ZS Vascular phenotype of amyloid precursor protein-deficient mice. Am J Physiol Heart Circ Physiol 316:H1297-H1308 (2019). PubMed: 30901278
  • Yao M  et al. CCR2 Chemokine Receptors Enhance Growth and Cell-Cycle Progression of Breast Cancer Cells through SRC and PKC Activation. Mol Cancer Res 17:604-617 (2019). PubMed: 30446625
  • Zaman RT  et al. Imaging cellular pharmacokinetics of 18F-FDG and 6-NBDG uptake by inflammatory and stem cells. PLoS One 13:e0192662 (2018). PubMed: 29462173
  • Villani R  et al. Dominant-negative Sox18 function inhibits dermal papilla maturation and differentiation in all murine hair types. Development 144:1887-1895 (2017). PubMed: 28512199
  • Madrigal-Matute J  et al. TWEAK/Fn14 interaction promotes oxidative stress through NADPH oxidase activation in macrophages. Cardiovasc Res 108:139-47 (2015). PubMed: 26224570

Customer reviews and Q&As

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Question

Dear Sir/Madam, We would like to know if this Anti-CPI17 alpha (phospho T38) antibody has been detected in C2C12 cellular extracts. If so, could you please send me some information regarding the detecting with this antibody. Looking forward to hearing from you.

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Abcam community

Verified customer

Asked on Oct 17 2012

Answer

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells.

https://www.abcam.com/CPI-17-phospho-T38-antibody-ab52174.html&tab=abreviews&intabreviewid=13947

In his treatment, this customer used oxytocin, which we carry as ab120186 and phorbol 12-myristate 13-acetate (PMA), which we carry as ab120297.

https://www.abcam.com/Oxytocin-ab120186.html

https://www.abcam.com/Phorbol-12-myristate-13-acetate-PMA-ab120297.html

I do not know enough about the protein to tell you definitively whether or not this protein is endogenously phosphorylated in C2C12 cells. I do have an image of ab52174 used in WB with untreated RAW264.7 cells though, which we sell as ab7187. I'll be sure to get that WB image added to the datasheet online, but it will take a few days to appear.

https://www.abcam.com/RAW-264-7-Mouse-leukaemic-monocyte-macrophage-cell-line-Whole-Cell-Lysate-ab7187.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Abcam Scientific Support

Answered on Oct 17 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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