RAW 264.7 whole cell lysate (ab7187)
Overview
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Product name
RAW 264.7 whole cell lysate -
General notes
Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor).
Growth media: DMEM and 10% fetal bovine serum.Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: 100% SDS Sample Buffer -
Concentration information loading... -
Lysate notes
Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. -
Research areas
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Background
Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent dilution. Ready to load.
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| Notes |
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WB
Use at an assay dependent dilution. Ready to load. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab7187 has been referenced in 8 publications.
- Manian KV et al. 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration. Cell Stem Cell 28:846-862.e8 (2021). PubMed: 33784497
- d'Uscio LV & Katusic ZS Endothelium-specific deletion of amyloid-β precursor protein exacerbates endothelial dysfunction induced by aging. Aging (Albany NY) 13:19165-19185 (2021). PubMed: 34382945
- Magalhaes RS et al. A tissue-engineered uterus supports live births in rabbits. Nat Biotechnol 38:1280-1287 (2020). PubMed: 32601434
- d'Uscio LV & Katusic ZS Vascular phenotype of amyloid precursor protein-deficient mice. Am J Physiol Heart Circ Physiol 316:H1297-H1308 (2019). PubMed: 30901278
- Yao M et al. CCR2 Chemokine Receptors Enhance Growth and Cell-Cycle Progression of Breast Cancer Cells through SRC and PKC Activation. Mol Cancer Res 17:604-617 (2019). PubMed: 30446625
- Zaman RT et al. Imaging cellular pharmacokinetics of 18F-FDG and 6-NBDG uptake by inflammatory and stem cells. PLoS One 13:e0192662 (2018). PubMed: 29462173
- Villani R et al. Dominant-negative Sox18 function inhibits dermal papilla maturation and differentiation in all murine hair types. Development 144:1887-1895 (2017). PubMed: 28512199
- Madrigal-Matute J et al. TWEAK/Fn14 interaction promotes oxidative stress through NADPH oxidase activation in macrophages. Cardiovasc Res 108:139-47 (2015). PubMed: 26224570