BCA protein assay kit for low concentrations (ab207002)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
Overview
-
Product name
BCA protein assay kit for low concentrations
See all BCA Protein kits -
Detection method
Colorimetric -
Sample type
Cell Lysate, Purified protein, Tissue Lysate -
Product overview
Abcam’s BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.
The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The Cu1+-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 µg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.
Support on how to determine protein concentration can be found here.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K814 Extra Sense™ BCA Protein Assay Kit. K814-2500 is the same size as the 2500 test size of ab207002.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at room temperature. Please refer to protocols. -
Components 2500 tests BCA Reagent A 1 x 190ml BCA Reagent B 1 x 190ml BCA Reagent C 1 x 9ml BSA 5 x 1ml -
Research areas
-
Relevance
The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration. -
Alternative names
- BCA
- Bicinchoninic acid
- Bicinchoninic acid protein
Associated products
-
Related Products
Images
-
Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).
Typical standard curve obtained for BSA and Cytochrome C from bovine heart using ab207002 and the microplate procedure (37°C for 2 hours).
-
Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).Typical standard curve obtained for BSA and Cytochrome C from bovine heart by using ab207002 and the test tube procedure (60°C for 1 hour).
Datasheets and documents
-
SDS download
-
Datasheet download
References (14)
ab207002 has been referenced in 14 publications.
- Fauzi M et al. Preservation effect of imeglimin on pancreatic β-cell mass: Noninvasive evaluation using 111In-exendin-4 SPECT/CT imaging and the perspective of mitochondrial involvements. Front Endocrinol (Lausanne) 13:1010825 (2022). PubMed: 36246910
- Gericke A et al. Sulodexide reduces glucose induced senescence in human retinal endothelial cells. Sci Rep 11:11532 (2021). PubMed: 34075143
- Wójtowicz J et al. Bean and Pea Plastoglobules Change in Response to Chilling Stress. Int J Mol Sci 22:N/A (2021). PubMed: 34769326
- Takov K et al. Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential. Basic Res Cardiol 115:26 (2020). PubMed: 32146560
- Wójtowicz J et al. Compensation Mechanism of the Photosynthetic Apparatus in Arabidopsis thaliana ch1 Mutants. Int J Mol Sci 22:N/A (2020). PubMed: 33379339