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    products/chip-kits/bca-protein-assaykit-ab102536.pdf

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Kits/ Lysates/ Other Tools and Reagents Western Blot Tools and Reagents Protein quantification kits
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BCA Protein Assay Kit (ab102536)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (14)References (136)

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BCA Protein Assay using ab102536
  • BCA Protein Assay using ab102536

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Assay time: 1 hr 30 min
  • Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts
  • Sensitivity: 0.5 µg

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Overview

  • Product name

    BCA Protein Assay Kit
    See all BCA Protein kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Quantitative
  • Sensitivity

    > 0.5 µg
  • Range

    0.5 µg - 30 µg
  • Assay time

    1h 30m
  • Product overview

    BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.


    The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).


    The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.


    The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 µg protein (0.01-0.6 mg/ml).


    Support on how to determine protein concentration can be found here.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1000 tests
    BCA Reagent NM 1 x 100ml
    BSA Standard Yellow 1 x 1ml
    Copper Reagent Blue 1 x 2ml
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Protein quantification kits
    • Kits/ Lysates/ Other
    • Kits
    • Molecular Biology Kits
    • Protein Quantitation Kits
    • BCA
  • Relevance

    The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration.
  • Alternative names

    • BCA
    • Bicinchoninic acid
    • Bicinchoninic acid protein

Images

  • BCA Protein Assay using ab102536
    BCA Protein Assay using ab102536
    Standard Curve at 60°C.
  • BCA Protein Assay using ab102536
    BCA Protein Assay using ab102536
    Standard Curve at 37°C.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (136)

Publishing research using ab102536? Please let us know so that we can cite the reference in this datasheet.

ab102536 has been referenced in 136 publications.

  • Xu J  et al. Carvedilol alleviates lipopolysaccharide (LPS)-induced acute lung injury by inhibiting Ras homolog family member A (RhoA)/ROCK activities. Bioengineered 13:4137-4145 (2022). PubMed: 35188451
  • Liu M  et al. miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1. Cardiol Res Pract 2022:7687154 (2022). PubMed: 35223094
  • Yao D  et al. circHIPK3 regulates cell proliferation and migration by sponging microRNA-124 and regulating serine/threonine kinase 3 expression in esophageal squamous cell carcinoma. Bioengineered 13:9767-9780 (2022). PubMed: 35443871
  • Chen X  et al. Circ_0014359 promotes oral squamous cell carcinoma progression by sponging miR-149. Acta Biochim Pol 69:315-320 (2022). PubMed: 35612554
  • Chi A  et al. ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress. Front Endocrinol (Lausanne) 13:871548 (2022). PubMed: 35634492
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 14 Abreviews or Q&A

Question

Will a 560nm absorbance filter be acceptable for this assay, given that the absorbance maxima is 562nm?

Read More

Abcam community

Verified customer

Asked on Jan 25 2012

Answer

Thank you for contacting us.

I have attached a graph of absorbance values measured at various wavelengths of light for various concentrations of BSA. The data demonstrates that the maximum absorbance is at 562nm but there is little loss of absorbance on either side of this peak, +/- 5nm, so your 560nm filter will be fine for this assay. In any case, it is likely that what you have is a band-pass filter centered on 560nm but allowing light above and below this wavelength to pass through, within a defined range that includes 562nm.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 25 2012

Question

Would the BCA assay kit actually do in case we used EDTA plasma or citrate plasma, instead of the heparin plasma? Or would there be some interference?

Read More

Abcam community

Verified customer

Asked on Oct 01 2013

Answer

It all depends on the concentration of EDTA and Citrate. For instance, EDTA<10mM and sodium citrate <200mM can be used with a standard BCA assay. Please be aware that the BCA assay is based on Copper ion chemistry, therefore it is not advised to use any metal chelator (such as EDTA at higher concentration of the one suggested).

Read More

Abcam Scientific Support

Answered on Oct 01 2013

Question

In order analyse our human serum and plasma (where heparin has been added as the coagulant) samples, which Total Protein Assay is more appropriate?

Read More

Abcam community

Verified customer

Asked on Sep 27 2013

Answer

I can confirm that both BCA and Bradford protein quantification kits can be used for human serum and plasma samples. The main difference between the two kits is that the BCA assay uses a Copper ion based chemistry whereas the Bradford assay uses a Coomassie-dye based reaction to produce color. The addition of heparin on your sample can form heparin-copper complex that can chelate out the copper ions in the BCA reagent. Therefore, in your particular case I will suggest to use the Bradford Assay (ab102535).

Read More

Abcam Scientific Support

Answered on Sep 27 2013

Question

I was just wondering if you any one knows whether the BCA quantitation kit (ab102536) is compatible with reducing agents such as DTT or BME?

If not would it be possible to confirm this with the supplier?

Best Regards,

Read More

Abcam community

Verified customer

Asked on Dec 04 2012

Answer

Thank you for contacting us.

If you are interested in knowing the compatibility of diffrent substances then following link will be helpful. They use the same compound for estimation as we use;

http://genome.yale.edu/images/a/aa/BCA.pdf


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Dec 04 2012

Question

Pourriez-vous nous indiquer la durée de vie de votre produit :

REF : ab10253, BCA Protein quantification kit.

Pourriez-vous également nous fournir le CoA de ce produit pour le lot GR48804-1 ?

En vous remerciant.

Read More

Abcam community

Verified customer

Asked on Nov 19 2012

Answer

Merci de nous avoir contactés.
Nos produits ne sont pas tous fabriqués en interne. Nous vendons des produits que nous pensons être d'intérêt pour nos clients qui proviennent d’autres fournisseurs ou collaborateurs universitaires. Tous ces produits sont garantis d’être de haute qualité et sont couvert par notre Abpromise. Notre Abpromise vous assure que si nos produits ne fonctionnent pas comme indiquer sur nos fiches techniques, nous vous offrirons un remplacement, un avoir ou un remboursement (sous réserve d'une commande ultérieure de 120 jours).
Certains de nos collaborateurs ne sont pas certifiés ISO9001 et donc nous ne sommes pas en mesure de fournir un certificat d'analyse pour ces produits. Comme l'identité de nos collaborateurs est confidentielle, nous fournissons un certificat de conformité (CoC) pour l'ensemble de nos produits disponibles dans notre catalogue. Ce document certifie que nos produits répondent aux spécifications requises pour un lot particulier.
Par conséquent, je suis ravie de vous envoyer un certificat de conformité pour BCA Protein Quantification Kit, ab102536 (voir pièce ci-jointe).
En ce qui concerne les dates d'expirations, nous ne donnons pas de date d'expiration pour nos produits. La plus part de nos produits sont stables et peuvent durer de quelques mois à plusieurs années s’ils sont conservés comme indique sur la fiche technique.Nous vous recommandons donc de suivre les instructions de stockage sur la fiche technique du produit que vous avez acheté. Ces conditions de stockage varieront pour tous nos produits et il est donc important de vérifier les conditions de stockage pour chacun de nos produits lorsque vous les recevez. Nous garantissons tous nos produits de fonctionner pour au moins 6 mois après la date d'achat s’ils sont stockés correctement.
Pour plus de détails sur notre garantie, visitez la page suivante de notre site:
https://www.abcam.com/index.html?pageconfig=abpromise
J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.
Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Nov 19 2012

Question

abcam gibt auf der Internetseite die Rezeptur für einen Tris-Triton Lysepuffer (Beginners Guide für Western Blots). Ich habe diesen einmal ausprobiert, finde aber keine Proteinbestimmungsmethode, die alle Inhaltsstoffe (EDTA und Triton-X) in den eingesetzten Größenordnungen toleriert und somit geeignet ist. Leider kann ich meine Proben auch nicht verdünnen, da die Proteingehalte der Proben per se sehr gering sind. Welche Methode zur Proteinbestimmung können Sie für diesen Lysepuffer empfehlen?



Ich hoffe, Sie können mir erneut weiterhelfen und bedanke mich für Ihre Mühe recht herzlich im Voraus.



Mit freundlichen Grüßen

Read More

Abcam community

Verified customer

Asked on Nov 12 2012

Answer

Vielen Dank für Ihre Anfrage.

Ich kann bestätigen, dass die meisten Methoden zur Proteinbestimmung nicht funktionieren wenn Detergenzien enthalten sind.

Die beste und robusteste Methode ist deshalb der BCA Protein Assay. Diese Methode ist kompatibel mit bis zu 5% Triton-X und 10mM EDTA.

Ich kann außerdem empfehlen, eine Positivkontrolle mit BSA im selben Puffer mitlaufen zu lassen.

Wir bieten den BCA Protein Assay in Form des folgenden Kits an:

ab102536

https://www.abcam.com/BCA-Protein-Assay-Kit-ab102536.html (or use the following: https://www.abcam.com/BCA-Protein-Assay-Kit-ab102536.html).

Ich hoffe, diese Information ist hilfreich und wünsche Ihnen viel Erfolg mit Ihren Versuchen.

Read More

Abcam Scientific Support

Answered on Nov 12 2012

Question

Hi,

Thanks for your suggestions. Actually, I am doing western for a transcription factor, which in literature is suggested to be having a very short half-life. So, an expert in the field suggested me to use Sample buffer directly to lyse the cells, as has been practised in his lab. But I don’t know, if I use RIPA buffer alone, maybe there is chance of proteolysis even with protease inhibitor and working on ice. But the thing is that there is no presence of debris after lysis with sample buffer after centrifugation at 14000xg / 15 min to clarify the supernatant, whereas with RIP alone we do get some debris, which off course is not a problem, as we take the supernatant mainly. Previously, I worked with tissues, and RIPA alone worked fine for me.

Looking forward to some valuable suggestions.

Best Regards,

Read More

Abcam community

Verified customer

Asked on Nov 08 2012

Answer

Thank you for your email and sorry for my late reply.

I think if an expert in the field has suggested this method has worked for his group it may well be worth trying. What you could always do is try both methods of lysis and probe the samples to see which method works best in your hands.

The quantification for the protein should not be a problem with the BCA kit. The reason why detergent can interfere with the assay is because higher concentrations of detergents tend to bind to proteins tightly and interfere with the final colour development. However, BCA based assays are very detergents tolerant. If you want to make sure that accurate readings are being taken, you could always dilute your samples lysed in the sample buffer by at least 2 in PBS or similar then take the reading. This will make the total detergent content below 5% and should therefore not have a significant affect on the assay.

I hope this information has been of help. If I can be of any further assistance, please do let me know.

Read More

Abcam Scientific Support

Answered on Nov 08 2012

Question

Dear xxxx,

I think I will stick to the order already placed with you, as the quality what I expected from another supplier is not up to the mark. Please try your level best to deliver the same at the earliest, that is what I can say at most.

In the BCA kit, while aliquoting the BSA, I found the volume of the stock to be about 1.1 ml instead of 1 ml as written on the label. Is it going to affect the concentration?

Another thing, I would like to get clarified as regards the storing of the HO-1 antibody, which is supplied in 50% glycerol, and has been recommended to be aliquoted. But I found that an antibody from another reputed supplier which is also supplied in glycerol, has not been recommended to be aliquoted. In glycerol, the antibody, remains in solution, so I think short freezing-thawing should not affect the quality. Is there any special reason, to keep the glycerol preserved antibody in aliquots?

Best Regards,

Read More

Abcam community

Verified customer

Asked on Oct 04 2012

Answer

Thank you for your reply.

I will then let the order through as it stands. We will endeavor to have the product with you as soon as possible but as I am sure you can understand we are reliant on delivery companies to move the package on our behalf. I will keep you updated with its progress.

As for the BCA kit and the extra BSA you have received compared to the 1 mL described. This is not unusual, we tend to supply a larger volume of all our products than we advertise to take into account any pipetting performed on our side. We want to make sure that the customer never receives less than expected and therefore typically over fill, with up to 10% of product. This should have no effect on the concentration of BSA supplied.

We do advise aliquotting the Anti-Heme Oxygenase 1 antibody when stored at -20°C regardless of the fact that it is in 50% glycerol. This is to avoid the antibody going through multiple freeze/thaw cycles (or more accurately with the glycerol added, temperature changes). This can affect the activity of the antibody and as a precautionary measure this is what we would advise. It also helps in that it can reduce the chance of contamination of the stock.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

Read More

Abcam Scientific Support

Answered on Oct 04 2012

Question

Hi Karin,

May be we can place order for more DTT later. But for the time being-

1. Please place order for one unit each of the following-

- ab13243 (rabbit polyclonal HO-1)

- ab102536 (BCA kit)

2. Please place order for 4 units of the following-

- ab119199 (DTT reducer)

Also, if you have any small inexpensive timer for sale, please let know, as I keep on losing my timer and would like to purchase one.

Our details-

MVH,

Read More

Abcam community

Verified customer

Asked on Oct 02 2012

Answer

Thank you for your reply.

I have now placed your order for you. This is on the order number 1xxxxand xxxx. The DTT reducer is currently not directly in stock and it will therefore take a few weeks to reach you. I have therefore split the orders. The anti-HO1 antibody and BCA kit(ab13243 and ab102536 respectively) will be with you tomorrow.

The total cost of the order is €757 (you are only being charged one shipping charge of €22 in this total).

We do not currently sell timers but I have included one of our promotional ones in your order for free.

I also have a little further information to share in regards to the DTT reducer. I have been advised that this reagent is likely to be stable for up to 3 months if stored as directed on the datasheet (4°C). It may therefore be better to order it as you need it rather than stock up.

I can also confirm that the 10x10 cm Optiblot gels will be compatible with the Dual Cool Electrophoresis system (DCX-700) you are using. The best kit for you may therefore be the Optiblot Reducing Electrophoresis Kit (10x10) at 12% or 4-20% (kit https://www.abcam.com/ab119218or https://www.abcam.com/ab119220respectively).

I hope this information has been of help.Please do let me know if I can be of any further assistance.

Read More

Abcam Scientific Support

Answered on Oct 02 2012

Question

Hi Karin,

Thanks. For the BCA kit, we are planning to use the Nanodrop machine. Is there any minituarised protocol for the available BCA kit to be used with Nanodrop or in microplate? If, so please let me know.

I will place order for HO-1 antibody and BCA kit by tomorrow.

Best Regards,

Debashish

Read More

Abcam community

Verified customer

Asked on Sep 28 2012

Answer

Thank you for your reply.

The protocol provided with the BCA kit (ab102536) describes how to use the kit with a microplate reader. I have attached this document for your information. It is possible the kit could be adapted to be used with a nanodrop reader but some optimisation may be required on your part as we have not performed this ourselves.

I hope this information has been of help. Let me know if I can be of any further assistance.

Read More

Abcam Scientific Support

Answered on Sep 28 2012

1-10 of 14 Abreviews or Q&A

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