Beta Secretase Activity Assay Kit (Fluorometric) (ab65357)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
Overview
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Product name
Beta Secretase Activity Assay Kit (Fluorometric)
See all Beta secretase kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Enzyme activity -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Beta Secretase Activity Assay Kit (ab65357) uses a convenient fluorescence method for detecting beta secretase activity in biological and purified samples.
The beta secretase assay protocol uses a secretase-specific peptide conjugated to two reporter molecules, EDANS and DABCYL. In the uncleaved form, the fluorescent signal from EDANS is quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by beta secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K360 Beta-Secretase Activity Fluorometric Assay Kit. K360-100 is the same size as the 100 test size of ab65357.
β-Secretase (Beta secretase) is responsible for the proteolytic process of the amyloid precursor protein (APP). It cleaves APP at the N-terminus of the A-beta peptide sequence, between residues 671 – 672, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer’s disease.
The standard is affinity purified.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components Identifier 100 tests β -Secretase Extraction Buffer NM 1 x 25ml β -Secretase Inhibitor (in DMSO) Blue 1 x 10µl β -Secretase Reaction Buffer (2X) WM 1 x 10ml Active β -Secretase Red 1 x 20µl β -Secretase Substrate (in DMSO) Amber NM 1 x 200µl -
Research areas
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Relevance
Beta secretase is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. Beta APP cleavage by beta-secretase results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Alternative names
- APP beta secretase
- ASP 2
- ASP2
see all
Associated products
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Related Products
Images
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Functional Studies - Beta Secretase Activity Assay Kit (Fluorometric) (ab65357)
Beta Secretase Activity measured in biological fluids. Tested neat.
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Functional Studies - Beta Secretase Activity Assay Kit (Fluorometric) (ab65357)Beta Secretase Activity measured in tissue lysates (each with protein concentration of 4 mg/mL)
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Functional Studies - Beta Secretase Activity Assay Kit (Fluorometric) (ab65357)Beta Secretase Activity measured in cell lysates (each with concentration of 2e7 cells/mL).
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Functional Studies - Beta Secretase Activity Assay Kit (Fluorometric) (ab65357)Beta-Secretase Cleavage Activity assayed using ab65357.
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab65357 has been referenced in 33 publications.
- Mohammad A et al. Voluntary wheel running alters markers of amyloid-beta precursor protein processing in an ovarian hormone depleted model. Front Endocrinol (Lausanne) 13:1069404 (2022). PubMed: 36561562
- Wang NY et al. Ferulic Acid Ameliorates Alzheimer's Disease-like Pathology and Repairs Cognitive Decline by Preventing Capillary Hypofunction in APP/PS1 Mice. Neurotherapeutics 18:1064-1080 (2021). PubMed: 33786807
- Baranowski BJ et al. Examination of BDNF Treatment on BACE1 Activity and Acute Exercise on Brain BDNF Signaling. Front Cell Neurosci 15:665867 (2021). PubMed: 34017238
- Sil S et al. Astrocytes & Astrocyte derived Extracellular Vesicles in Morphine Induced Amyloidopathy: Implications for Cognitive Deficits in Opiate Abusers. Aging Dis 12:1389-1408 (2021). PubMed: 34527417
- Hata S et al. Suppression of amyloid-β secretion from neurons by cis-9, trans-11-octadecadienoic acid, an isomer of conjugated linoleic acid. J Neurochem 159:603-617 (2021). PubMed: 34379812