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Cell Plasma Membrane Staining Kit - Deep Red Fluorescence - Cytopainter (ab219942)

  • Datasheet
  • SDS
  • Protocol Booklet
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CytoPainter Cell Plasma Membrane Staining Kit - Deep Red Fluorescence (ab219942)

    Key features and details

    • Assay type: Cell-based
    • Detection method: Fluorescent
    • Platform: Fluorescence microscope
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      Cell Plasma Membrane Staining Kit - Deep Red Fluorescence - Cytopainter
      See all Cell Plasma Membrane kits
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Cell Plasma Membrane Staining Kit - Deep Red Fluorescence | Cytopainter (ab219942) uses a deep red cell membrane probe (Ex/Em = 640/660 nm) that enables the uniform staining of cell membrane across a wide variety of mammalian cell types. The kit provides an excellent tool for the rapid staining of plasma membranes in suspended or attached live cells depending on the cell type and experimental conditions. The fluorescence staining in cell membranes is also maintained after fixation with formaldehyde, enabling further multi-color staining. In addition, the kit provides robust and flexible staining in live and fixed cells, and can be adapted for many different types of fluorescence platforms, such as fluorescence imaging and flow cytometry.


      Review other dyes and kits for plasma membrane staining, or the live cell staining fluorescent dyes guide

    • Notes

      The cell membrane (plasma membrane) is a thin semi-permeable membrane that separates the interior of all cells from the environment. The basic function of the cell membrane is to protect the cell from its surroundings. It is composed mainly of lipids and proteins. Cell membranes are involved in a variety of cellular processes such as cell adhesion, ion conductivity and cell signaling and serve as the attachment surface for several extracellular structures, including the cell wall, glycocalyx, and intracellular cytoskeleton.

    • Platform

      Fluorescence microscope

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 500 tests
      Assay Buffer 1 x 50ml
      Deep Red Dye 1 vial
      DMSO 1 x 200µl

    Images

    • CytoPainter Cell Plasma Membrane Staining Kit - Deep Red Fluorescence (ab219942)
      CytoPainter Cell Plasma Membrane Staining Kit - Deep Red Fluorescence (ab219942)

      HeLa cells stained with CytoPainter Cell Plasma Membrane Staining Kit – Deep Red Fluorescence (ab219942) in a 96 well clear-bottom plate.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab219942? Please let us know so that we can cite the reference in this datasheet.

    ab219942 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Excellent cell steining

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    I used Cytopainter to stain chondrocytes. it was easy, quick and bright! did not see any background off target fluorescence.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 09 2021

    Membrane staining in Neuroblastama cell line N2A

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    It is friendly product in use. One drawback is that also some cytoplasm fragment is stained.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Łukasz Witucki

    Verified customer

    Submitted Mar 12 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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