ChIP Kit Magnetic - One Step (ab156907)
Key features and details
- Assay time: 5 hr
Overview
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Product name
ChIP Kit Magnetic - One Step
See all ChIP Kit kits -
Assay time
5h 00m -
Product overview
Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
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Notes
Primers
The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.
ChIP assay products and guides
Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.
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Tested applications
Suitable for: ChIPmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Strip Caps 1 x 12 units 1 x 24 units 96-Well PCR Plate 1 x 2 units 1 x 2 units Anti-RNA Polymerase II (1 mg/mL) 1 x 8µl 1 x 16µl ChIP Buffer 1 x 6ml 1 x 12ml DNA Release Buffer 1 x 14ml 1 x 28ml GAPDH Primer - Forward (20 µM) 1 x 8µl 1 x 16µl GAPDH Primer - Reverse (20 µM) 1 x 8µl 1 x 16µl Magnetic Beads 1 x 220µl 1 x 450µl Non-Immune IgG (1 mg/mL) 1 x 10µl 1 x 20µl Proteinase K (10 mg/mL) 1 x 55µl 1 x 110µl -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired. |
| Notes |
|---|
|
ChIP
Use at an assay dependent concentration. The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired. |
Images
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Typical Results
The data shows the analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit Magnetic - One Step (ab156907) with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab156907 has been referenced in 10 publications.
- Su W et al. Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer. J Exp Clin Cancer Res 40:88 (2021). PubMed: 33648545
- Guo F et al. NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15. Cell Death Discov 7:78 (2021). PubMed: 33850096
- Zhang K et al. NR5A2 Is One of 12 Transcription Factors Predicting Prognosis in HNSCC and Regulates Cancer Cell Proliferation in a p53-Dependent Manner. Front Oncol 11:691318 (2021). PubMed: 34277436
- He H et al. USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway. Int J Biol Sci 17:2417-2429 (2021). PubMed: 34326684
- Yang M et al. OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation. Cell Rep 30:4165-4178.e7 (2020). PubMed: 32209476
- Yang C et al. FBP1 binds to the bromodomain of BRD4 to inhibit pancreatic cancer progression. Am J Cancer Res 10:523-535 (2020). PubMed: 32195024
- Zhang B et al. The CDK4/6 inhibitor PD0332991 stabilizes FBP1 by repressing MAGED1 expression in pancreatic ductal adenocarcinoma. Int J Biochem Cell Biol 128:105859 (2020). PubMed: 32987196
- Fan P et al. Overexpressed histone acetyltransferase 1 regulates cancer immunity by increasing programmed death-ligand 1 expression in pancreatic cancer. J Exp Clin Cancer Res 38:47 (2019). PubMed: 30709380
- Yang G et al. VDAC1 is regulated by BRD4 and contributes to JQ1 resistance in breast cancer. Oncol Lett 18:2340-2347 (2019). PubMed: 31452730
- Kim TH & Cho SG Melatonin-induced KiSS1 expression inhibits triple-negative breast cancer cell invasiveness. Oncol Lett 14:2511-2516 (2017). PubMed: 28781689