Chromatin Extraction Kit - Flexible Format (ab223876)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Chromatin Extraction Kit - Flexible Format
See all Chromatin Extraction kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
Chromatin Extraction Kit (ab223876) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Extraction and Lysis Buffer 1 x 100ml Formaldehyde Quenching Solution (10X) 1 x 50ml IP Buffer Concentrate (11X) 1 x 5ml Protease Inhibitor Cocktail 1 x 0.5ml Stringency supplement 1 x 5ml -
Research areas
Images
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Analysis of chromatin fragmentation following 15min of sonication
Chromatin was prepared from 10×106 of cross-linked HeLa cells (human cervical cancer) using ab223876 (left lane) or competitor’s kit (right lane) as per corresponding manuals. Chromatin fragmentation was analysed using standard method. For ChIP experiments chromatin must be sheared to fragments < 1000 bp.
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Analysis of chromatin fragmentation following 15min of sonicationChromatin was prepared from 10×106 cells of cross-linked cells or 30 mg of tissue using extraction and lysis buffer. Left to right: Mw markers, HeLa (human cervical cancer), A549 (human lung carcinoma), F9 (mouse 129 embryo teratocarcinoma), mouse liver.
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Analysis of chromatin fragmentation following 15min of sonicationChromatin was prepared from various amount of cross-linked HeLa cells (human cervical cancer) resuspended in 0.5 mL of extraction and lysis buffer. Left to right: 10×106, 5×106, 2×106, 1×106, 1×105 cells per prep.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223876 has not yet been referenced specifically in any publications.