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Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)

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Functional Studies - Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)

    Key features and details

    • Assay type: Enzyme activity
    • Platform: Microplate reader
    • Assay time: 1 hr

    Overview

    • Product name

      Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit
      See all KDM1 / LSD1 kits

    • Assay type

      Enzyme activity

    • Assay time

      1h 00m

    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Mammals

    • Product overview

      Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456) allows the user to measure inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for screening KDM1/LSD1 inhibitors, which are directly interacting with he enzyme or blocking the binding to its substrate.


      KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.


      This kit may cross react with LSD2.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 48 tests 96 tests
      8-Well Assay Strips (with Frame) 6 units 12 units
      Fluoro Developer 1 x 12µl 1 x 24µl
      Fluoro Diluter 1 x 4ml 1 x 8ml
      Fluoro Enhancer 1 x 12µl 1 x 24µl
      LSD1 Assay Buffer 1 x 2ml 1 x 4ml
      LSD1 Assay Standard, 100 mM 1 x 10µl 1 x 20µl
      LSD1 Inhibitor, 1 mM 1 x 25µl 1 x 50µl
      LSD1 Substrate, 0.7 mM 1 x 150µl 1 x 300µl

    • Research areas

    • Relevance

      Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.

    • Alternative names

      • Amine oxidase (flavin containing) domain 2
      • AOF2
      • BHC110
      • BRAF35 HDAC complex protein BHC110
      • BRAF35-HDAC complex protein BHC110
      • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
      • Flavin-containing amine oxidase domain-containing protein 2
      • KDM1
      • KIAA0601
      • LSD1
      • Lysine (K) specific demethylase 1
      • Lysine (K) specific demethylase 1A
      • Lysine specific histone demethylase 1
      • Lysine specific histone demethylase 1A
      • Lysine-specific histone demethylase 1
      • Lysine-specific histone demethylase 1A
      see all

    • Database links

      Images

      • Functional Studies - Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)
        Functional Studies - Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)
        Inhibition of KDM1/LSD1 by tranylcypromine (TCP) detected using ab113456. [LSD1 concentration: 500 ng/well].

      References (0)

      Publishing research using ab113456? Please let us know so that we can cite the reference in this datasheet.

      ab113456 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Question

      I am working for Dana Farber Cancer Institute, Boston, MA.Our labis looking for a ELISA kit which can measure the activity of LSD1.For this, we are trying to understand the difference between all the 4 ELISA kits for LSD1(named below) ABcam is providing. 1. EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) 2. EpiSeeker KDM1/LSD1 Activity Quantification Kit (Colorimetric) ab113459 3. EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456) 4. EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460) Pleaselet us know the differentpurposes of each kit andhow to choose the correct kit for our experiments. Thanks in advace

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      Answer

      Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a colorimetric detection systems, whereas, ab113460 has a fluorescent detections method. Depending on the equipment available in your lab, would determine which one of these you would choose. The ab113457 measures histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria and this is an indirect detection method, compared to the above kits. For ab113456, this kit is ready-to-use and provides all the essential components needed for screening KDM1/LSD1 inhibitors, which are directly interacting with the enzyme or blocking the binding to its substrate. You can find more information about these products and how to choose the correct kit for your needs, by clicking on the following link: https://www.abcam.com/index.htmlpageconfig=resource&rid=14469&source=pagetrap&viapagetrap=episeeker#3 If there is anything else I can help you with, please let me know.

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