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Histone H3 Acetylation Assay Kit (ab115102)

Reviews (2)Q&A (5)References (6)

Key features and details

  • Assay type: Cell-based (quantitative)
  • Platform: Microplate reader
  • Assay time: 5 hr
  • Sample type: Adherent cells, Suspension cells, Tissue
  • Sensitivity: 1 ng

Overview

  • Product name

    Histone H3 Acetylation Assay Kit
    See all Histone H3 acetylation kits

  • Sample type

    Tissue, Adherent cells, Suspension cells

  • Assay type

    Cell-based (quantitative)

  • Sensitivity

    1 ng

  • Range

    1 ng - 100 ng

  • Assay time

    5h 00m

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals

  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.

     Histone H3 Acetylation Assay Kit (ab115102) allows the user to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Platform

    Microplate reader

Properties

References (6)

Publishing research using ab115102? Please let us know so that we can cite the reference in this datasheet.

ab115102 has been referenced in 6 publications.

  • Ortiz AM  et al. Butyrate administration is not sufficient to improve immune reconstitution in antiretroviral-treated SIV-infected macaques. Sci Rep 12:7491 (2022). PubMed: 35523797
  • McAlpine CS  et al. Sleep exerts lasting effects on hematopoietic stem cell function and diversity. J Exp Med 219:N/A (2022). PubMed: 36129517
  • Srinivasan SP  et al. Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells. Cell Death Discov 8:447 (2022). PubMed: 36335090
  • Schwarz A  et al. Induction of Regulatory T Cells and Correction of Cytokine Disbalance by Short-Chain Fatty Acids: Implications for Psoriasis Therapy. J Invest Dermatol 141:95-104.e2 (2021). PubMed: 32544478
  • Chen CL  et al. Arginine is an epigenetic regulator targeting TEAD4 to modulate OXPHOS in prostate cancer cells. Nat Commun 12:2398 (2021). PubMed: 33893278
  • Svoboda P  et al. Nuclear transport of nicotinamide phosphoribosyltransferase is cell cycle-dependent in mammalian cells, and its inhibition slows cell growth. J Biol Chem 294:8676-8689 (2019). PubMed: 30975903

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Histone H3 Acetylation in Zebrafish embryos

 Average Good 4/5 (Ease of Use)
Abreviews
abreview image
Sample Type: Histone from zebrafish embryos at 3hpf.
The assay procedure was made follow the manufacture´s instructions, using 200 ng/ul of histone extract. The positive control (H3 acetilated control) was diluted 1:1000. For the trial we included the positive and negative control (Histone buffer) and the diluted histone sample.
The results obtained after measure the absorbance at 450 nm are shown in the image.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 08 2017

Histone H3 Acetylation in Zebrafish Testicle Cells

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Sample Type: Cells dissociated from zebrafish testicle.
Extraction of histones: the protocol described in the datasheed did not work well. The difficult was to resuspended the tissue (or cells when dissociated) in the Lysis Buffer. When the tissue was resuspended appeared a mucus material that made impossible to resuspend well the tissue.
For that reason, we chose another methology (used routinely in our lab): dissociated tissue was subjected to hypotonic lysis buffer (10mM Tris-Cl pH 8.0 1mM KCl 1.5 mM MgCl2 1mM DTT) and the obtained pellet was solubilized in HCl 0.4 N (to obtain the basic proteins). After this incubation step, we proceed with a common precipitation with TCA/Acetone and the final protein pellet was solubilized in molecular biology water. With a starting material of 2·10^8 cel/mL we obtained aproximately 6000 ug/mL of histones.
Assay procedure: the histones obtained as was described before were diluted to 300 ng/uL and were used as the protocol described. For the trial we included a negative control (water), a positive control (H3 Acetilated Control at 15 ng/uL) and the diluted histone sample.
Results of absorbance at 450 nm are shown in the image below.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Silvia González Rojo

Verified customer

Submitted Jan 26 2017

Question

What is the difference between ab115102 and ab115124?

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Answer

Compared to ab115102, ab115124 is much faster and easier to handle with higher sensitivity and better reproducibility, but slightly less specificity. 1-2 ug of histone protein (min 50,000 cells) may be needed for each assay point if using ab115102, while only 50-100 ng of histone protein (min 5000 cells) are required for each point with use of ab115124. For both kits, the CV% is less than 8% between the wells and less than 12% between the strips.

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Question

Base on the same principles the ab115102 may be suitable to use in fish too?



Also I'm interesting in ab133099 and ab113476 it may funtion?

Read More
Answer

Hello! The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable. Please let me know if you have further questions.

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Question

I have been looking at your assays for assessing global H3 and H4 histone acetylation (e.g ab115102). I would like to assess acetylation in tissue samples that have already been lysed in RIPA buffer. I notice that these kits have their own lysis buffer and a histone extraction step and I wondered if they would be suitable for samples in RIPA buffer?

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Answer

I have discussed your question with some colleagues in the Lab and can confirm that RIPA buffer would not be compatible with the 10X Lysis Buffer.

If needed, you can purchase the 10X Lysis Buffer component individually from us.

Read More

Question

I am trying to figure out with of your H3 Acetylation kits would work best for me, but I am having a difficult time figuring out the difference between:
EpiSeeker Histone H3 Acetylation Assay Kit (ab115102)
EpiSeeker Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)
As they both measure H3 total acetylation via colorimetric means. Please explain what I am missing. Furthermore, do you have any information to indicate that these kits may work in yeast. Thank you.

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Answer

>The kits are very similar. The main differences are that the ab115102 requires 5 hrs to run and you need to coat the antibody yourself while the kit ab115124 only requires 2.5 hours and the wells come pre-coated.

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Question

I have a question regarding the EpiSeeker Histone H3 and H4 Acteylation Assay Kits (ab115102, ab115103). The kit instructions indicate that I will need a fluorescent microplate reader and a 60 or 100 mm plate. We do have a fluorescent microplate reader but it works only on 96 well plates. Will this be okay?

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Answer

Regarding the use of a 60 or 100mm plate - this is used for disecting tissue samples if you are using them, however, once you proceed with the histone extraction, the assay is then performed in the strip wells provided with the kit. The strips can be put in the plate frame to analyze on the microplate reader.

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