Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell Lysate, Tissue Extracts
Product nameHistone H3 (K27) Methyltransferase Activity Quantification Assay Kit
See all Histone Methyltransferase kits
Sample typeTissue Extracts, Cell Lysate
Assay time3h 00m
Species reactivityReacts with: Mouse, Human
Predicted to work with: Mammals
Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.
Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. In mammalian cells, the EZ family group of proteins among others are responsible for the addition of one, two or three methyl groups at lysine 17 of histone H3.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 10X Wash Buffer 1 x 11ml 1 x 22ml 8-Well Assay Strips (with Frame) 6 units 12 units Adomet 1 x 25µl 1 x 50µl Biotinylated Substrate, 25 µg/mL 1 x 100µl 1 x 200µl Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Control Enzyme (300 µg/ml) 1 x 5µl 1 x 10µl Detection Antibody, 100 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 6ml 1 x 12ml Histone Assay Buffer 1 x 1.5ml 1 x 3ml HMT Standard, 10 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml
Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3K27 specific histone methyltrasferase activity was measured.
ab113454 has been referenced in 1 publication.
- Hyman G et al. Epigenetic approaches in glioblastoma multiforme and their implication in screening and diagnosis. Methods Mol Biol 1238:511-21 (2015). PubMed: 25421677