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Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)

Reviews (1)Q&A (5)References (7)
Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)
  • Functional Assay: ab115124 Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)
  • Functional Assay: ab115124 Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 2 hr 30 min
  • Sample type: Adherent cells, Suspension cells, Tissue
  • Sensitivity: 2 ng

Overview

  • Product name

    Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)
    See all Histone H3 acetylation kits

  • Detection method

    Colorimetric

  • Sample type

    Tissue, Adherent cells, Suspension cells

  • Sensitivity

    2 ng

  • Range

    2 ng - 100 ng

  • Assay time

    2h 30m

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals

  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
    Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) allows the user to colorimetrically detect and quantify if histone H3 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115124 is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Platform

    Microplate reader

Properties

Images

  • Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)
    Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)Nowacka-Woszuk, Joanna et al., PloS one?vol. 13,2 e0193464., Fig 4, doi:10.1371/journal.pone.0193464

    The differences in H3 acetylation level in fetal livers (female fetuses aged 19 dpc) from F1, F2, and F3 generations. measured with ab113476. Histones were prepared using ab115124
    Asterisk indicate a significant difference between the respective C and R groups: ∗∗ P < 0.01; NS not significant. Data presented as the means ± SE (n = 6 per group).

  • Functional Assay: ab115124 Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)
    Functional Assay: ab115124 Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)

    Acetylation Histone 3 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 20-500 ng per well) (duplicates +/- SD).

  • Functional Assay: ab115124 Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)
    Functional Assay: ab115124 Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)

    Standard curve with background signal subtracted (duplicates; +/- SD).

References (7)

Publishing research using ab115124? Please let us know so that we can cite the reference in this datasheet.

ab115124 has been referenced in 7 publications.

  • Moronczyk J  et al. Insights into the Histone Acetylation-Mediated Regulation of the Transcription Factor Genes That Control the Embryogenic Transition in the Somatic Cells of Arabidopsis. Cells 11:N/A (2022). PubMed: 35269485
  • Casati L  et al. Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration. Int J Mol Sci 21:N/A (2020). PubMed: 32629979
  • Zhao B  et al. The BET-bromodomain inhibitor JQ1 mitigates vemurafenib drug resistance in melanoma. Melanoma Res 28:521-526 (2018). PubMed: 30192303
  • Nowacka-Woszuk J  et al. Transgenerational effects of prenatal restricted diet on gene expression and histone modifications in the rat. PLoS One 13:e0193464 (2018). PubMed: 29474484
  • Zhang D  et al. miR-185 mediates lung epithelial cell death after oxidative stress. Am J Physiol Lung Cell Mol Physiol 310:L700-10 (2016). PubMed: 26747785
  • Kocic G  et al. Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease. Ren Fail 36:1078-82 (2014). Functional Studies ; Human . PubMed: 24845033
  • Dong G  et al. Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows. PLoS One 9:e107659 (2014). Functional Studies ; Cow . PubMed: 25222274

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Histone H3 total acetylation detection in a non-model insect

 Good Excellent 5/5 (Ease of Use)
Abreviews
abreview image
This assay was performed on histones isolated from the flesh fly, Sarcophaga bullata. Histones were isolated from diapause and control pupae using the Histone Extraction kit (ab113476) and had been stored at -80 °C for approximately 6 months before being used in the assay. The total protein for each sample was estimated by measuring absorbance at 280 nm with a nanodrop spectrophotometer. All samples were diluted to 1 µg of protein using the Antibody buffer provided with the kit. A range of protein amounts (1-10 µg) were used in the assay.
The assay was conducted according to the provided instructions. Samples and standards were incubated at 25 °C for ~ 100 minutes before proceeding with the first wash step. Average, blank corrected, O.D. values for acetylated standards ranged from 0.221 to 5.86 the curve was approximately logarithmic (see below). The average, blank corrected, O.D. values ranged from 0.014 to 0.1 for control samples and 0.006 to 0.283 for diapause samples. Only the 10 µg per well diapause samples had O.D. values within the range of the standards. The control samples appeared to have ~ 50 % less acetylated histone protein than the diapause samples (see figure below), as was expected from our previous research.
In summary, the kit was easy to use and does appear to work with our non-model insect.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Julie Reynolds

Verified customer

Submitted Feb 08 2016

Question

Why as in Enhancer required for use in ab115125 and not with ab115124?

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Answer



The detection antibody in ab115124 kit is labeled with signal reporter already. But H4 antibody included in the kit ab115125 is not and needs to be labeled by signal reporter and enhanced with enhancer during the assay.

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Question

I want to detect potential changes in histone acetylation in different tissues in mice after treatment with different types of substances with HDAC inhibitory actions and I'm considering using your Episeeker kits for isolation of histones and quantification of changes in histone acetylation.

I have a few questions about these procedures:

1. Is it possible to freeze pieces of tissue/cell pellets before running them in the histone extraction kit?

2. Since I'm using mice and want to look at histones not only from tissue but also from leukocytes I will have limited number of starting cells (probably < 10^6). Is this a problem? Would it be better to pool blood to get more cells?

3. Which method/protocol do you recommend for quantifying protein/histones after the histone extraction procedure to make sure equal loading of sample in the histone H3 or H4 total acetylation detection kit?

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Answer



1. Yes, it is possible for tissue or cell pellets to be frozen before extracting histone proteins.

2. The minimum amount of histone protein required as input for these assays are 50 ng (both for the colorimetric and fluorometric kits). In principle, 50 ng of histone proteins could be obtained from as little as 100,000 cells so pooling of blood may not be necessary. However we recommend using 1 million cells if possible in order to obtain a suitable amount of histone proteins for the assay.

3. We recommend using any standard protein quantification assay such as a Bradford assay for histone quantification.

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Question

What is the difference between ab115102 and ab115124?

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Answer

Compared to ab115102, ab115124 is much faster and easier to handle with higher sensitivity and better reproducibility, but slightly less specificity. 1-2 ug of histone protein (min 50,000 cells) may be needed for each assay point if using ab115102, while only 50-100 ng of histone protein (min 5000 cells) are required for each point with use of ab115124. For both kits, the CV% is less than 8% between the wells and less than 12% between the strips.

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Question

I'm interesting in buy some histone assay kits like: ab115103, ab115124 and others. The Kit stablish cross reactivity for mammals. I wondering if it may function with zebrafish tissues. Please let me know if I can use it with fish tissue or if you have other recomendations I will apreciate it.

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Answer

Thank you for contacting us. We have not tested these kits in fish and have no testing data on this use. The kit ab115103 may be suitable for use in fish based on principle. However, the kit ab115124 may not be suitable for fish.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I am trying to figure out with of your H3 Acetylation kits would work best for me, but I am having a difficult time figuring out the difference between:
EpiSeeker Histone H3 Acetylation Assay Kit (ab115102)
EpiSeeker Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124)
As they both measure H3 total acetylation via colorimetric means. Please explain what I am missing. Furthermore, do you have any information to indicate that these kits may work in yeast.

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Answer

The kits are very similar. The main differences are that the ab115102 requires 5 hrs to run and you need to coat the antibody yourself while the kit ab115124 only requires 2.5 hours and the wells come pre-coated.

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