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Epigenetics and Nuclear Signaling Histones H3 Unmodified
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Histone H3 Total Quantification Kit (Fluorometric) (ab115092)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional Studies - Histone H3 Quantification Assay Kit (ab115092)

    Key features and details

    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Assay time: 2 hr 30 min
    • Sample type: Adherent cells, Suspension cells, Tissue
    • Sensitivity: 2 ng/well

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    Overview

    • Product name

      Histone H3 Total Quantification Kit (Fluorometric)
      See all Histone H3 kits
    • Detection method

      Fluorescent
    • Sample type

      Tissue, Adherent cells, Suspension cells
    • Sensitivity

      2 ng/well
    • Range

      10 ng/well - 1000 ng/well
    • Assay time

      2h 30m
    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Mammals
    • Product overview

      Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.

      Histone H3 Total Quantification Kit (Fluorometric) (ab115092) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components Identifier 48 tests 96 tests
      10X Wash Buffer 1 x 10ml 1 x 20ml
      8 well Sample Strips (with frame) 4 units 9 units
      8 well Standard Control Strips Green Ringed 2 units 3 units
      Antibody Buffer 1 x 6ml 1 x 12ml
      Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl
      Fluoro Developer 1 x 12µl 1 x 24µl
      Fluoro Dilution 1 x 4ml 1 x 8ml
      Fluoro Enhancer 1 x 12µl 1 x 24µl
      Standard Control (100 µg/mL) 1 x 10µl 1 x 20µl
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Unmodified
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Methylated
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Ubiquitinated
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Acetylated
      • Epigenetics and Nuclear Signaling
      • Histones
      • H3
      • Phosphorylated
      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • Histone methylation quantification
      • Epigenetics and Nuclear Signaling
      • Assays & Kits
      • Histone methylation quantification
    • Relevance

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • Cellular localization

      Nucleus. Chromosome.
    • Alternative names

      • H3f3a
      • H3FA
      • H3FB
      • H3FC
      • H3FD
      • H3FF
      • H3FH
      • H3FI
      • H3FJ
      • H3FK
      • H3FL
      • HIST1H3A
      • HIST1H3B
      • HIST1H3C
      • HIST1H3D
      • HIST1H3E
      • HIST1H3F
      • HIST1H3G
      • HIST1H3H
      • HIST1H3I
      • HIST1H3J
      • HIST3H3
      • HIST3HA
      • Histone H3.1
      • Histone H3.2
      • Histone H3.3
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/i
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      see all
    • Database links

      • Entrez Gene: 8350 Human
      • Entrez Gene: 8351 Human
      • Entrez Gene: 8352 Human
      • Entrez Gene: 8353 Human
      • Entrez Gene: 8354 Human
      • Entrez Gene: 8355 Human
      • Entrez Gene: 8356 Human
      • Entrez Gene: 8357 Human
      • Entrez Gene: 8358 Human
      • Entrez Gene: 8968 Human
      • Entrez Gene: 319152 Mouse
      • Entrez Gene: 319153 Mouse
      • Entrez Gene: 360198 Mouse
      • Entrez Gene: 97908 Mouse
      • Entrez Gene: 100365669 Rat
      • Entrez Gene: 291159 Rat
      • Entrez Gene: 679994 Rat
      • Entrez Gene: 680511 Rat
      • Entrez Gene: 682330 Rat
      • Omim: 601128 Human
      • SwissProt: P84243 Human
      • SwissProt: P68431 Human
      • SwissProt: Q71DI3 Human
      • SwissProt: Q16695 Human
      • SwissProt: Q93081 Human
      • SwissProt: P84244 Mouse
      • SwissProt: P68433 Mouse
      • SwissProt: P84228 Mouse
      • SwissProt: Q6LED0 Rat
      see all

    Associated products

    • Related Products

      • Histone H3 (di-methyl K4) Quantification Kit (Colorimetric) (ab115052)
      • Histone H3 (di-methyl K4) Quantification Kit (Fluorometric) (ab115053)
      • Histone H3 (mono-methyl K4) Quantification Kit (Colorimetric) (ab115054)
      • Histone H3 (mono-methyl K4) Quantification Kit (Fluorometric) (ab115055)
      • Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056)
      • Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057)
      • Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric) (ab115058)
      • Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059)
      • Histone H3 Total Quantification Kit (Colorimetric) (ab115091)

    Images

    • Functional Studies - Histone H3 Quantification Assay Kit (ab115092)
      Functional Studies - Histone H3 Quantification Assay Kit (ab115092)

      A histone H3 standard curve was generated as described in the protocol of the Histone H3 Total Quantification Kit (Fluorometric) (ab115092).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab115092? Please let us know so that we can cite the reference in this datasheet.

    ab115092 has not yet been referenced specifically in any publications.

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