Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)

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Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)

    Key features and details

    • Assay type: Enzyme activity
    • Platform: Microplate reader
    • Assay time: 3 hr
    • Sample type: Cell Lysate, Tissue Extracts

    Overview

    • Product name

      Histone Demethylase (H3K4) Activity Quantification Assay Kit

    • Sample type

      Tissue Extracts, Cell Lysate

    • Assay type

      Enzyme activity

    • Assay time

      3h 00m

    • Species reactivity

      Reacts with: Mouse, Human, Plants
      Predicted to work with: Mammals, Other species

    • Product overview

      Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455) allows the user to measure activity/inhibition of H3K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K4 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.


      Methylation of histone H3 at lysine 4 (H3K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3K4 demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3K4, and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a hydroxylation reaction.


       

    • Platform

      Microplate reader

    Properties

    Images

    • Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)
      Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)

      Nuclear extracts were prepared from Hela cells using the ab113475 and H3K4 specific histone demethyase activity was measured.

    References (1)

    Publishing research using ab113455? Please let us know so that we can cite the reference in this datasheet.

    ab113455 has been referenced in 1 publication.

    • Jamshidi S  et al. KDM5B protein expressed in viable and fertile ?ARID mice exhibit no demethylase activity. Int J Oncol 59:N/A (2021). PubMed: 34713299

    Customer reviews and Q&As

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    Question

    We would like to quantify H3K4me3 demethylation activity in mouse embryonic stem cell extracts. You have two kits listed that seem to both serve that purpose: 113464 and 113455. - What is the difference between these two? - Do you recommend one over the other for K4me3 demethylation? - Has any of them been used in any publications?

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    Answer

    Thank you for your interest in ab113455 & ab113464.

    You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimethylated histone H3-K4 substrate while the substrate used in ab113464 is a trimethylated histone H3-K4 substrate.

    Due to these different substrates, ab113455 will detect LSD1 type demethylase activity which can remove mono and dimethyl groups while ab113464 will detect JARID type demethylase activity which removes trimethyl groups from H3 K4.

    So it seems ab113464 would be the proper choice for your interest in studying H3K4me3 demethylation activity.

    As far as we know neither of these kits have been used in published works.

    I hope this is helpful. Please contact us again if you have any further questions.

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