KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr
Product nameKDM1/LSD1 Activity Quantification Assay Kit (Fluorometric)
See all KDM1 / LSD1 kits
Assay typeEnzyme activity
Assay time3h 00m
Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: Mammals, Other species
KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3 (meK4), a specific tag for epigenetic transcriptional activation.
KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460) is designed for the easy and fast fluorometric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
This kit may cross react with LSD2.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl Fluoro Developer 1 x 10µl 1 x 20µl Fluoro Diluter 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 10µl 1 x 20µl LSD1 Assay Buffer 1 x 4ml 1 x 8ml LSD1 Assay Standard, 50 µg/mL 1 x 10µl 1 x 20µl LSD1 Inhibitor Tranylcypromine, 1 mM 1 x 20µl 1 x 40µl LSD1 Substrate, 50 µg/ml 1 x 60µl 1 x 120µl
RelevanceHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
- Amine oxidase (flavin containing) domain 2
- Entrez Gene: 23028 Human
- Entrez Gene: 99982 Mouse
- Omim: 609132 Human
- SwissProt: O60341 Human
- SwissProt: Q6ZQ88 Mouse
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Datasheets and documents
ab113460 has not yet been referenced specifically in any publications.