KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr
Overview
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Product name
KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric)
See all KDM4/JMJD2 kits -
Detection method
Fluorescent -
Assay type
Enzyme activity -
Assay time
3h 00m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.
KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462) is designed for the easy and fast fluorometric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
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Notes
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Co-factor 1 1 x 30µl 1 x 60µl Co-factor 2 1 x 30µl 1 x 60µl Co-factor 3 1 x 30µl 1 x 60µl Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl Fluoro Developer 1 x 10µl 1 x 20µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 10µl 1 x 20µl JMJD2 Assay Buffer 1 x 4ml 1 x 8ml JMJD2 Assay Standard, 50 µg/mL 1 x 10µl 1 x 20µl JMJD2 Substrate, 50 µg/mL 1 x 60µl 1 x 120µl -
Research areas
Associated products
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Related Products
- KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
- KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460)
- KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461)
- KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463)
- KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)
- Nuclear Extraction Kit (ab113474)
Images
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Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113462.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab113462 has been referenced in 2 publications.
- Xiang K et al. Accumulation of oncometabolite D-2-Hydroxyglutarate by SLC25A1 inhibition: A metabolic strategy for induction of HR-ness and radiosensitivity. Cell Death Dis 13:641 (2022). PubMed: 35869047
- Jin C et al. Chem-seq permits identification of genomic targets of drugs against androgen receptor regulation selected by functional phenotypic screens. Proc Natl Acad Sci U S A 111:9235-40 (2014). PubMed: 24928520