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    products/chip-kits/kdm4jmjd2-activity-quantification-assay-kit-fluorometric-ab113462.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (2)

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Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)

    Key features and details

    • Assay type: Enzyme activity
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Assay time: 3 hr

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    Overview

    • Product name

      KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric)
      See all KDM4/JMJD2 kits
    • Detection method

      Fluorescent
    • Assay type

      Enzyme activity
    • Assay time

      3h 00m
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Mammals
    • Product overview

      KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.


      KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462) is designed for the easy and fast fluorometric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

    • Notes

       

       

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 48 tests 96 tests
      10X Wash Buffer 1 x 14ml 1 x 28ml
      8-Well Assay Strips (with Frame) 6 units 12 units
      Adhesive Covering Film 1 unit 1 unit
      Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl
      Co-factor 1 1 x 30µl 1 x 60µl
      Co-factor 2 1 x 30µl 1 x 60µl
      Co-factor 3 1 x 30µl 1 x 60µl
      Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl
      Fluoro Developer 1 x 10µl 1 x 20µl
      Fluoro Dilution 1 x 4ml 1 x 8ml
      Fluoro Enhancer 1 x 10µl 1 x 20µl
      JMJD2 Assay Buffer 1 x 4ml 1 x 8ml
      JMJD2 Assay Standard, 50 µg/mL 1 x 10µl 1 x 20µl
      JMJD2 Substrate, 50 µg/mL 1 x 60µl 1 x 120µl
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Jumonji containing proteins
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Methylation
      • Lysine demethylation
      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • Histone demethylation
      • Epigenetics and Nuclear Signaling
      • Assays & Kits
      • DNA modification and methylation

    Associated products

    • Related Products

      • KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
      • KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460)
      • KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461)
      • KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463)
      • KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)
      • Nuclear Extraction Kit (ab113474)

    Images

    • Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)
      Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)
      Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113462.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab113462? Please let us know so that we can cite the reference in this datasheet.

    ab113462 has been referenced in 2 publications.

    • Xiang K  et al. Accumulation of oncometabolite D-2-Hydroxyglutarate by SLC25A1 inhibition: A metabolic strategy for induction of HR-ness and radiosensitivity. Cell Death Dis 13:641 (2022). PubMed: 35869047
    • Jin C  et al. Chem-seq permits identification of genomic targets of drugs against androgen receptor regulation selected by functional phenotypic screens. Proc Natl Acad Sci U S A 111:9235-40 (2014). PubMed: 24928520

    Customer reviews and Q&As

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    Question



    This order was for the Abcam product EpiSeeker KDM4/JMJD2 Activity Quantification Assay kit (Fluorometric) Cat log no: AB113462. The kit has 14 components, but we only received four of the components EJM3, EJM4, EJM6 and EJM7.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 12 2012

    Answer

    Thank you for contacting Abcam.

    I am sorry about the kit shortages and I have asked for a newkit to be sent free of charge to replace the existing one.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Mar 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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