KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
Overview
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Product name
KDM5/JARID Activity Quantification Assay Kit (Colorimetric)
See all KDM5/JARID kits -
Detection method
Colorimetric -
Assay type
Enzyme activity -
Assay time
3h 00m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.
KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463) is designed for the easy and fast colorimetric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Co-factor 1 1 x 30µl 1 x 60µl Co-factor 2 1 x 30µl 1 x 60µl Co-factor 3 1 x 30µl 1 x 60µl Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl Developer Solution 1 x 5ml 1 x 10ml JARID Assay Buffer 1 x 4ml 1 x 8ml JARID Assay Standard, 50 µg/mL 1 x 10µl 1 x 20µl JARID Substrate, 50 µg/mL 1 x 60µl 1 x 120µl Stop Solution 1 x 5ml 1 x 10ml -
Research areas
Associated products
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Related Products
- KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
- KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460)
- KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461)
- KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462)
- KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)
- Nuclear Extraction Kit (ab113474)
Images
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Functional Studies - KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463)Demonstration of high sensitivity of the KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113463.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab113463 has been referenced in 2 publications.
- Khan MI et al. Molecular profiling of epigenetic landscape of cancer cells during extracellular matrix detachment. Sci Rep 11:2784 (2021). PubMed: 33531586
- Khan MI et al. AKT Inhibition Modulates H3K4 Demethylase Levels in PTEN-Null Prostate Cancer. Mol Cancer Ther 18:356-363 (2019). PubMed: 30446585