Neutrophil Elastase Inhibitor Assay Kit (ab118971)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 40 min
Overview
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Product name
Neutrophil Elastase Inhibitor Assay Kit
See all Neutrophil Elastase kits -
Detection method
Fluorescent -
Assay type
Quantitative -
Assay time
0h 40m -
Product overview
In Abcam's Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K782 Neutrophil Elastase Inhibitor Screening Kit (Fluorometric). K782-100 is the same size as the 100 test size of ab118971.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Inhibitor Control (3 mM, SPCK) Purple 1 x 100µl Neutrophil Elastase Green 1 vial Substrate Red 1 x 0.2ml -
Research areas
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Relevance
Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage. -
Alternative names
- Bone marrow serine protease
- ELA2
- ELANE
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Images
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Neutrophil Elastase Inhibitor Screening Kit (ab118971)
Neutrophil Elastase Activity Profile at Various Concentrations of Elafin/Skalp (ab153768)
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Neutrophil Elastase Inhibitor Screening Kit (ab118971)Neutrophil Elastase Activity Profile at Various Concentrations of SERPINB1 (ab114774)
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Functional Studies - Neutrophil Elastase Inhibitor Screening Kit (ab118971)Neutrophil Elastase Inhibition by SPCK -
Functional Studies - Neutrophil Elastase Inhibitor Screening Kit (ab118971)Neutrophil Elastase Activity Profile at Various Concentrations of SPCK
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab118971 has been referenced in 11 publications.
- Bujak T et al. Flower Extracts as Multifunctional Dyes in the Cosmetics Industry. Molecules 27:N/A (2022). PubMed: 35164187
- Ziemlewska A et al. Evaluation of Cosmetic and Dermatological Properties of Kombucha-Fermented Berry Leaf Extracts Considered to Be By-Products. Molecules 27:N/A (2022). PubMed: 35408743
- Zagórska-Dziok M et al. Positive Effect of Cannabis sativa L. Herb Extracts on Skin Cells and Assessment of Cannabinoid-Based Hydrogels Properties. Molecules 26:N/A (2021). PubMed: 33557174
- Bujak T et al. Antioxidant and Cytoprotective Properties of Plant Extract from Dry Flowers as Functional Dyes for Cosmetic Products. Molecules 26:N/A (2021). PubMed: 34068613
- Ziemlewska A et al. Effect of fermentation time on the content of bioactive compounds with cosmetic and dermatological properties in Kombucha Yerba Mate extracts. Sci Rep 11:18792 (2021). PubMed: 34552134