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  1. Link

    products/chip-kits/phalloidin-ifluor-488-reagent-ab176753.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Phalloidin-iFluor 488 Reagent (ab176753)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (14) Submit a question References (215)

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Whole Mount Immunofluorescence of Mouse Inner Ear
  • Phalloidin iFluor 488 staining of macrophages
  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)
  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

Key features and details

  • Assay type: Cell-based (qualitative)
  • Platform: Fluorescence microscope
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Phalloidin-iFluor 488 Reagent
    See all F-actin kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be easily detected with a fluorescent microscope at Ex/Em = 493/517 nm.


    Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used with paraffin-embedded samples that have been de-paraffinized.


    Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

  • Notes

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 300 tests
    Phalloidin-iFluor 488 Conjugate 1 x 300 tests
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • F-actin Staining Kits
  • Alternative names

    • actin filament
    • f actin
    • Filamentous actin

Images

  • Whole Mount Immunofluorescence of Mouse Inner Ear
    Whole Mount Immunofluorescence of Mouse Inner EarImage courtesy of Mr. Shahar Taiber

    Ab176753 at 1/1000 dilution in PBS whole mount Immunofluorescence of Mouse inner ear sensory epithelia. Tissue was incubated for 2 hours at room temperature.

  • Phalloidin iFluor 488 staining of macrophages
    Phalloidin iFluor 488 staining of macrophagesReview by Dr. Jose Ramos Vivas

    Macrophage J774A.1 cell line stained with Phalloidin 488 and DAPI nuclear counterstaining.

  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)
    CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

    Actin filaments staining in HeLa cells. Actin filaments (green) were stained with CytoPainter Phalloidin-iFluor 488 reagent (ab176753); tubulin filaments were stained with a mouse anti-tubulin antibody/goat anti-mouse IgG (red). Nuclei were stained with Hoechst 33342.

  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)
    CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

    Excitation and emission spectra of phalloidin-iFluor 488 reagent.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (215)

Publishing research using ab176753? Please let us know so that we can cite the reference in this datasheet.

ab176753 has been referenced in 215 publications.

  • Yuan X  et al. Reciprocal interaction between vascular niche and sweat gland promotes sweat gland regeneration. Bioact Mater 21:340-357 (2023). PubMed: 36185745
  • Viloria K  et al. GC-Globulin/Vitamin D-Binding Protein Is Required for Pancreatic α-Cell Adaptation to Metabolic Stress. Diabetes 72:275-289 (2023). PubMed: 36445949
  • Samant RS  et al. Native Size-Exclusion Chromatography-Based Mass Spectrometry Reveals New Components of the Early Heat Shock Protein 90 Inhibition Response Among Limited Global Changes. Mol Cell Proteomics 22:100485 (2023). PubMed: 36549590
  • Hang PZ  et al. Small-molecule 7,8-dihydroxyflavone counteracts compensated and decompensated cardiac hypertrophy via AMPK activation. J Geriatr Cardiol 19:853-866 (2022). PubMed: 36561053
  • Xiong H  et al. Easily attainable and low immunogenic stem cells from exfoliated deciduous teeth enhanced the in vivo bone regeneration ability of gelatin/bioactive glass microsphere composite scaffolds. Front Bioeng Biotechnol 10:1049626 (2022). PubMed: 36568292
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 14 Abreviews or Q&A

Great cell stain

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
We used our standard operating protocol for the DuaLink NETRI chip. The dye is convenient to use as it can be done with DAPI incubation. Specific and very bright, it stains with good definition primary human keratinocytes and hiPSC-derived sensory neurons.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Melanie Gleyzes

Verified customer

Submitted Feb 28 2023

Phalloidin-FITC

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Tested on A549 cells, dilution 1:2000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 19 2021

Phalloidin for IF of various human cells in culture

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Has worked for a variety of cells in culture (BeWo choriocarcinoma, HTR8-SVNeo cells) with ease. Permeabilization using 0.1% TritonX-100 in PBS for 5 min necessary prior to incubation with phalloidin (1:1000 in 0.1%BSA in PBS) for 20 min at room temperature.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 21 2021

Phalloidin iFluor 488 staining of equine tenocytes

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Images of unstimulated equine adult tenocytes on gelatin-coated coverslips. Cells were fixed in 4% PFA, permeabilized with Triton X (0.1%) for 60 min, and blocked in 2.5% horse serum for 20 min at room temperature. Subsequently, actin was stained with Ab176753 at 1/1000 dilution in 2.5% horse serum for 90 min at room temperature. Nuclear counterstaining with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ross Beaumont

Verified customer

Submitted May 18 2021

lab Manager

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
The reagent works
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 27 2019

CytoPainter Phalloidin-iFluor 488 labeling of plant material

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Detection of F-actin in live pollen of 3 species: Gagea lutea, Saintpaulia ionatha and Yucca filamentosa:

Pollen from - 20 degrees Celsius were placed in 4 degrees Celsius for a night.
Next day placed in PBS buffer for 5 min and transferred into 1xPhalloidin-iFluor 488 working solution (0,5 ul + 500 ul PBS buffer) for 20 or 90 min at RT in darkness.
Immediately visualized using Leica DM6000B with FITC filter.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 16 2019

Phalloidin-iFluor 488 Reagent

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Bronchial epithelial cell line. 4% paraformaldehyde fixation for 15min RT. Stain with Phalloidin (1:1000) for 45 min at RT and nucleus co-stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 10 2019

Good staining

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Epithelial cells:
- Fixed 4% PFA (room temperature)
- Dilution 1:1000
- DAPI co-staining
- Fluorescence measured with confocal microscopy
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 24 2019

Whole mount IF for the inner ear

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
I used abcam's phalloidin-488 for whole mount IF of mouse inner ear (sensory epithelia). It stains actin so stereocilia are stained very strong. Diluted 1:1000 in PBS and incubated for 2 hours in RT.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Shahar Taiber

Verified customer

Submitted Jun 04 2018

Phalloidin immunofluorescence of lim2099 cells

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Seeding of 200.000 cells/well on cover slips in a 24-well-plate overnight.
5 min washing with PBS 0.1% Tween
20 min fixation with 4% PFA at room temperature.
3 x 5 min washing with PBS 0.1% Tween
10 min permeabilization with PBS 0.3% Triton-X 100
3 x 5 min washing with PBS 0.1% Tween.
Phalloidin staining for 1 hour at room temperature in the dark: 100 µl of 1x reagent diluted in PBS 0.1% Triton-X 100 1.5% BSA per coverslip.
3 x 5 min PBS 0.1% Tween.
Mounting of coverslips with ProLong Gold Antifade Mountant with DAPI on microscope slides.
Drying overnight at 4°C and imaging on the next day.

CytoPainter Phalloidin-iFluor 488 Reagent is an excellent stain yielding great results at low expenses in comparison to other more expensive stains from competing companies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 29 2017

1-10 of 14 Abreviews or Q&A

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