Phalloidin-iFluor 594 Reagent (ab176757)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Phalloidin-iFluor 594 Reagent
See all F-actin kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Phalloidin-iFluor 594 Reagent (ab176757) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-Fluor 594 can be detected with a fluorescent microscope at Ex/Em = 590/618 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
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Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Phalloidin-iFluor 594 Conjugate 1 x 300 tests -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
Images
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Whole Mount of Mouse Inner Ear stained with Phalloidin 594Image courtesy of Mr. Shahar Taiber
Ab176757 at 1/1000 dilution in PBS used for a whole-mount of the sensory epithelium in a mouse inner ear.
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CytoPainter Phalloidin-iFluor 594 Reagent (ab176757)Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 594 reagent (ab176757); ER (green) was stained with CytoPainter ER Staining Kit – Green Fluorescence (ab139481). Nuclei (blue) were stained with DAPI.
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Functional Studies - Phalloidin-iFluor 594 Reagent (ab176757)Sommerova, Lucia et al., BMC cancer vol. 17,1 546., Fig 3, doi:10.1186/s12885-017-3537-5The A549 KOAGR2 were stained with CytoPainter phalloidin-iFluor 594 reagent to detect morphological changes associated with F-actin reorganization. Nuclei were visualised with DAPI.
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CytoPainter Phalloidin-iFluor 594 Reagent (ab176757)Excitation and emission spectra of phalloidin-iFluor 594 reagent.
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab176757 has been referenced in 61 publications.
- Wang Q et al. Heterogeneity of human corneal endothelium implicates lncRNA NEAT1 in Fuchs endothelial corneal dystrophy. Mol Ther Nucleic Acids 27:880-893 (2022). PubMed: 35141048
- Chattopadhyaya S et al. Regulation of Cardiac Fibroblast GLS1 Expression by Scleraxis. Cells 11:N/A (2022). PubMed: 35563778
- Cui Z et al. Network Pharmacology Deciphers the Action of Bioactive Polypeptide in Attenuating Inflammatory Osteolysis via the Suppression of Oxidative Stress and Restoration of Bone Remodeling Balance. Oxid Med Cell Longev 2022:4913534 (2022). PubMed: 35578727
- Kim E et al. Fibroadipogenic Progenitors Regulate the Basal Proliferation of Satellite Cells and Homeostasis of Pharyngeal Muscles via HGF Secretion. Front Cell Dev Biol 10:875209 (2022). PubMed: 35669512
- Kim OV et al. Contribution of septins to human platelet structure and function. iScience 25:104654 (2022). PubMed: 35832887