Plasmin Activity Assay Kit (Fluorometric) (ab204728)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Plasma, Purified protein
- Sensitivity: 10 ng
Overview
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Product name
Plasmin Activity Assay Kit (Fluorometric)
See all Plasmin kits -
Detection method
Fluorescent -
Sample type
Plasma, Purified protein -
Assay type
Enzyme activity -
Sensitivity
10 ng -
Assay time
0h 20m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Plasmin Activity Assay Kit (Fluorometric) (ab204728) is based on the ability of Plasmin to proteolytically cleave a synthetic plasmin substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers at Ex/Em = 360/450 nm. This assay kit is simple, rapid and can detect plasmin activity as low as 10 ng in a variety of samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K381 Plasmin Activity Assay Kit (Fluorometric). K381-100 is the same size as the 100 test size of ab204728.
Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasminogen-Plasmin system has also been implicated in a wide variety of physiologic and pathologic processes, including tumor growth, invasion and metastasis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Plasmin Assay Buffer 1 x 15ml Plasmin Dilution Buffer 1 x 1.5ml Plasmin Enzyme Standard (1 mg/ml) 1 x 5µl Plasmin Substrate 1 x 200µl -
Research areas
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Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
Tissue specificity
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
Involvement in disease
Plasminogen deficiency -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Domain
Kringle domains mediate interaction with CSPG4. -
Post-translational
modificationsN-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
Cellular localization
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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Alternative names
- Plasmin light chain B
- Plasminogen
- PLG
- PLMN_HUMAN
Images
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Plasmin Activity Assay Kit (Fluorometric) (ab204728)
Typical Plasmin standard curve.
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Plasmin Activity Assay Kit (Fluorometric) (ab204728)Plasmin activity was measured in plasma samples in the presence and absence of a Plasmin inhibitor, Aprotinin. S = Substrate, I = Inhibitor, AB = Activation Buffer containing Urokinase
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab204728 has been referenced in 2 publications.
- Demers-Mathieu V et al. Premature delivery impacts the concentration of plasminogen activators and a plasminogen activator inhibitor and the plasmin activity in human milk. Front Pediatr 10:917179 (2022). PubMed: 36016873
- Cai M et al. Spinosin Attenuates Alzheimer's Disease-Associated Synaptic Dysfunction via Regulation of Plasmin Activity. Biomol Ther (Seoul) 28:131-136 (2020). PubMed: 31791115