Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Plasma, Purified protein
- Sensitivity: 10 ng
Product namePlasmin Activity Assay Kit (Fluorometric)
See all Plasmin kits
Sample typePlasma, Purified protein
Assay typeEnzyme activity
Assay time0h 20m
Species reactivityReacts with: Mammals, Other species
Plasmin Activity Assay Kit (Fluorometric) (ab204728) is based on the ability of Plasmin to proteolytically cleave a synthetic plasmin substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers at Ex/Em = 360/450 nm. This assay kit is simple, rapid and can detect plasmin activity as low as 10 ng in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K381 Plasmin Activity Assay Kit (Fluorometric). K381-100 is the same size as the 100 test size of ab204728.
Plasmin (EC 220.127.116.11) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasminogen-Plasmin system has also been implicated in a wide variety of physiologic and pathologic processes, including tumor growth, invasion and metastasis.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Plasmin Assay Buffer 1 x 15ml Plasmin Dilution Buffer 1 x 1.5ml Plasmin Enzyme Standard (1 mg/ml) 1 x 5µl Plasmin Substrate 1 x 200µl
FunctionPlasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
Tissue specificityPresent in plasma and many other extracellular fluids. It is synthesized in the liver.
Involvement in diseasePlasminogen deficiency
Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain.
DomainKringle domains mediate interaction with CSPG4.
modificationsN-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
Cellular localizationSecreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
- Information by UniProt
- Plasmin light chain B
ab204728 has been referenced in 2 publications.
- Demers-Mathieu V et al. Premature delivery impacts the concentration of plasminogen activators and a plasminogen activator inhibitor and the plasmin activity in human milk. Front Pediatr 10:917179 (2022). PubMed: 36016873
- Cai M et al. Spinosin Attenuates Alzheimer's Disease-Associated Synaptic Dysfunction via Regulation of Plasmin Activity. Biomol Ther (Seoul) 28:131-136 (2020). PubMed: 31791115